PMID- 22833358
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20130318
LR  - 20211021
IS  - 0920-9069 (Print)
IS  - 1573-0778 (Electronic)
IS  - 0920-9069 (Linking)
VI  - 65
IP  - 3
DP  - 2013 May
TI  - Establishment and cytogenetic characterization of a cell line from a pulmonary 
      metastasis of osteosarcoma.
PG  - 347-53
LID - 10.1007/s10616-012-9487-5 [doi]
AB  - Osteosarcoma (OS) is the most frequent malignant bone tumour in children and 
      adolescents. In metastatic patients, the most common site of metastasis is the 
      lung. There are relatively few cell lines of metastatic OS reported in the 
      literature and the cytogenetic aspects of OS metastases are still controversial 
      and inconclusive. Here we describe the establishment of a new OS cell line, M-OS, 
      from a pulmonary metastasis of a typical osteoblastic OS of an 11-year-old boy 
      with metastatic OS at diagnosis. M-OS cells have been maintained in culture for 
      over 50 passages for more than 1 year. M-OS was characterized by 
      immunohistochemistry, conventional cytogenetics and fluorescence in situ 
      hybridization (FISH). In order to evaluate in vitro cell modification, the 
      immunohistochemical analysis was performed in three different moments of the cell 
      line: 10th, 30th and 50th passages. The conventional cytogenetic analysis 
      revealed the ploidy of M-OS cell line as near-diploid, with most metaphases 
      hyperdiploid and tetraploid. We found a copy number gain of MDM2 gene as the most 
      frequent alteration in the FISH analysis. The immunohistochemical analysis 
      confirmed that M-OS cell line maintained the osteogenic nature even after all 
      passages for the cell line establishment in vitro.
FAU - Salinas-Souza, Carolina
AU  - Salinas-Souza C
AD  - Genetics Laboratory, Department of Pediatrics, Pediatric Oncology Institute 
      (IOP/GRAACC/UNIFESP), Federal University of Sao Paulo, Rua Botucatu, 743-8 degrees  
      andar, Sao Paulo, SP, 04023-062, Brazil.
FAU - Oliveira, Indhira Dias
AU  - Oliveira ID
FAU - de Oliveira, Renato
AU  - de Oliveira R
FAU - de Seixas Alves, Maria Teresa
AU  - de Seixas Alves MT
FAU - Petrilli, Antonio Sergio
AU  - Petrilli AS
FAU - Toledo, Silvia Regina Caminada
AU  - Toledo SR
LA  - eng
PT  - Journal Article
DEP - 20120726
PL  - United States
TA  - Cytotechnology
JT  - Cytotechnology
JID - 8807027
PMC - PMC3597172
EDAT- 2012/07/27 06:00
MHDA- 2012/07/27 06:01
PMCR- 2014/05/01
CRDT- 2012/07/27 06:00
PHST- 2012/02/22 00:00 [received]
PHST- 2012/07/10 00:00 [accepted]
PHST- 2012/07/27 06:00 [entrez]
PHST- 2012/07/27 06:00 [pubmed]
PHST- 2012/07/27 06:01 [medline]
PHST- 2014/05/01 00:00 [pmc-release]
AID - 9487 [pii]
AID - 10.1007/s10616-012-9487-5 [doi]
PST - ppublish
SO  - Cytotechnology. 2013 May;65(3):347-53. doi: 10.1007/s10616-012-9487-5. Epub 2012 
      Jul 26.