PMID- 22835519
OWN - NLM
STAT- MEDLINE
DCOM- 20130426
LR  - 20231002
IS  - 1095-9319 (Electronic)
IS  - 0026-2862 (Linking)
VI  - 84
IP  - 3
DP  - 2012 Nov
TI  - LIF maintains progenitor phenotype of endothelial progenitor cells via 
      Kruppel-like factor 4.
PG  - 270-7
LID - S0026-2862(12)00147-1 [pii]
LID - 10.1016/j.mvr.2012.07.005 [doi]
AB  - BACKGROUND: Endothelial progenitor cells (EPCs) participate in post-natal 
      vasculogenesis. Maintaining the preliminary progenitor phenotype and good 
      proliferation capacity of EPCs is key to their use in treating cardiovascular 
      ischemic diseases. However, transcriptional regulation in EPCs remains largely 
      unknown. We investigated the effect of leukemia inhibitory factor (LIF) combined 
      with vascular endothelial growth factor (VEGF) on EPCs and the potential roles of 
      Kruppel-like transcription factors (KLFs). METHODS AND RESULTS: Co-treatment with 
      LIF and VEGF (100 ng/ml each) (V+L) could increase EPC colony-forming units and 
      CD34 expression, which reflects the EPC progenitor phenotype and alleviated 
      differentiation of EPCs. The effect was associated with Akt activation and 
      increased expression of KLF4. Upregulation of KLF4 induced by V+L could be 
      inhibited by transfection with dominant-negative Akt adenovirus. Furthermore, 
      overexpression of KLF4 in EPCs enhanced the expression of CD34 and alleviated 
      cell differentiation but did not increase the phosphorylation of Akt. 
      CONCLUSIONS: LIF combined with VEGF can maintain the preliminary, progenitor 
      phenotype of EPCs and alleviate cell differentiation by upregulating KLF4, which 
      may provide new insights into transcriptional regulation in EPCs.
CI  - Copyright (c) 2012 Elsevier Inc. All rights reserved.
FAU - Li, Xiaoxia
AU  - Li X
AD  - Department of Physiology and Pathophysiology, Peking University Health Science 
      Center, Beijing, 100191 China. lixiaoxia@bjmu.edu.cn
FAU - Song, Yimeng
AU  - Song Y
FAU - Wang, Dawei
AU  - Wang D
FAU - Fu, Chenglai
AU  - Fu C
FAU - Zhu, Zhenjiu
AU  - Zhu Z
FAU - Han, Yingying
AU  - Han Y
FAU - Li, Chenghong
AU  - Li C
FAU - Wang, Nanping
AU  - Wang N
FAU - Zhu, Yi
AU  - Zhu Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120723
PL  - United States
TA  - Microvasc Res
JT  - Microvascular research
JID - 0165035
RN  - 0 (Antigens, CD34)
RN  - 0 (KLF4 protein, human)
RN  - 0 (Kruppel-Like Factor 4)
RN  - 0 (Kruppel-Like Transcription Factors)
RN  - 0 (Leukemia Inhibitory Factor)
RN  - 0 (Vascular Endothelial Growth Factor A)
SB  - IM
MH  - Antigens, CD34/biosynthesis
MH  - Cell Differentiation
MH  - Cell Proliferation
MH  - Endothelial Cells/*cytology
MH  - Fetal Blood/cytology
MH  - Flow Cytometry/methods
MH  - *Gene Expression Regulation
MH  - Humans
MH  - Kruppel-Like Factor 4
MH  - Kruppel-Like Transcription Factors/*metabolism
MH  - Leukemia Inhibitory Factor/*metabolism
MH  - Microcirculation
MH  - Phenotype
MH  - Phosphorylation
MH  - Stem Cells/*cytology
MH  - Vascular Endothelial Growth Factor A/metabolism
EDAT- 2012/07/28 06:00
MHDA- 2013/04/27 06:00
CRDT- 2012/07/28 06:00
PHST- 2011/09/09 00:00 [received]
PHST- 2012/06/22 00:00 [revised]
PHST- 2012/07/16 00:00 [accepted]
PHST- 2012/07/28 06:00 [entrez]
PHST- 2012/07/28 06:00 [pubmed]
PHST- 2013/04/27 06:00 [medline]
AID - S0026-2862(12)00147-1 [pii]
AID - 10.1016/j.mvr.2012.07.005 [doi]
PST - ppublish
SO  - Microvasc Res. 2012 Nov;84(3):270-7. doi: 10.1016/j.mvr.2012.07.005. Epub 2012 
      Jul 23.