PMID- 2283561
OWN - NLM
STAT- MEDLINE
DCOM- 19910315
LR  - 20190510
IS  - 0887-8013 (Print)
IS  - 0887-8013 (Linking)
VI  - 4
IP  - 6
DP  - 1990
TI  - Measurement of soluble MHC class I molecules in renal graft patients: a 
      noninvasive allograft monitor.
PG  - 426-9
AB  - We investigated the biochemistry of naturally occurring major histocompatibility 
      complex (MHC) class I molecules in human serum and established a quantitative 
      enzyme-linked immunoassay (ELISA) to determine soluble human leukocyte antigen 
      (HLA) concentration. Peptides of 46, 40, 37, 35, and 12 kDa were isolated on 
      affinity chromatography columns using two monoclonal antibodies (MoAbs), the 
      anti-heavy chain W6/32 and the anti-beta 2-microglobulin BBM. 1. These peptides 
      were confirmed on Western blots by the HC-10 MoAb, which binds a monomorphic 
      epitope on denatured heavy chains. In detergent-binding experiments, only the 
      46-kDa peptide could be isolated from solubilized cell membranes. No quantitative 
      differences between serum and plasma HLAs of the same individual were measured. 
      Soluble HLA expression in 12 renal graft recipients was measured over 1-3 mo 
      posttransplantation. A highly significant increase of 50%-100% was noted during 
      rejection episodes. Clinical signs of rejection were accompanied by poor renal 
      function, including elevated creatinine values. After the crises had been 
      managed, class I levels normalized to 0.3-1.5 micrograms/ml, which is the range 
      in healthy persons. Patients who showed no rejection crises maintained constant 
      levels within the period of study. We anticipate the application of soluble HLA 
      measurement in clinical practice as a noninvasive graft monitor.
FAU - Zavazava, N
AU  - Zavazava N
AD  - Department of Immunology, University of Kiel, Germany.
FAU - Leimenstoll, G
AU  - Leimenstoll G
FAU - Muller-Ruchholtz, W
AU  - Muller-Ruchholtz W
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Clin Lab Anal
JT  - Journal of clinical laboratory analysis
JID - 8801384
RN  - 0 (Histocompatibility Antigens Class I)
SB  - IM
MH  - Enzyme-Linked Immunosorbent Assay/*methods
MH  - Graft Rejection/immunology
MH  - Histocompatibility Antigens Class I/*blood/chemistry
MH  - Humans
MH  - Kidney Transplantation/*immunology
MH  - Molecular Weight
MH  - Time Factors
EDAT- 1990/01/01 00:00
MHDA- 1990/01/01 00:01
CRDT- 1990/01/01 00:00
PHST- 1990/01/01 00:00 [pubmed]
PHST- 1990/01/01 00:01 [medline]
PHST- 1990/01/01 00:00 [entrez]
AID - 10.1002/jcla.1860040607 [doi]
PST - ppublish
SO  - J Clin Lab Anal. 1990;4(6):426-9. doi: 10.1002/jcla.1860040607.