PMID- 22837074
OWN - NLM
STAT- MEDLINE
DCOM- 20121226
LR  - 20211021
IS  - 1552-4930 (Electronic)
IS  - 1552-4922 (Print)
IS  - 1552-4922 (Linking)
VI  - 81
IP  - 9
DP  - 2012 Sep
TI  - Image cytometry-based detection of aneuploidy by fluorescence in situ 
      hybridization in suspension.
PG  - 776-84
LID - 10.1002/cyto.a.22101 [doi]
AB  - Cytogenetic abnormalities are important diagnostic and prognostic criteria for 
      hematologic malignancies. Karyotyping and fluorescence in situ hybridization 
      (FISH) are the conventional methods by which these abnormalities are detected. 
      The sensitivity of these microscopy-based methods is limited by the abundance of 
      the abnormal cells in the samples and therefore these analyses are commonly not 
      applicable to minimal residual disease (MRD) stages. A flow cytometry-based 
      imaging approach was developed to detect chromosomal abnormalities following FISH 
      in suspension (FISH-IS), which enables the automated analysis of several 
      log-magnitude higher number of cells compared with the microscopy-based 
      approaches. This study demonstrates the applicability of FISH-IS for detecting 
      numerical chromosome aberrations, establishes accuracy, and sensitivity of 
      detection compared with conventional FISH, and feasibility to study procured 
      clinical samples of acute myeloid leukemia (AML). Male and female healthy donor 
      peripheral blood mononuclear cells hybridized with combinations of chromosome 
      enumeration probes (CEP) 8, X, and Y served as models for disomy, monosomy, and 
      trisomy. The sensitivity of detection of monosomies and trisomies amongst 20,000 
      analyzed cells was determined to be 1% with a high level of precision. A high 
      correlation (R(2) = 0.99) with conventional FISH analysis was found based on the 
      parallel analysis of diagnostic samples procured from 10 AML patients with 
      trisomy 8 (+8). Additionally, FISH-IS analysis of samples procured at the time of 
      clinical remission demonstrated the presence of residual +8 cells indicating that 
      this approach may be used to detect MRD and associated chromosomal defects.
CI  - Copyright (c) 2012 International Society for Advancement of Cytometry.
FAU - Minderman, Hans
AU  - Minderman H
AD  - Flow and Image Cytometry Laboratory, Department of Pathology and Laboratory 
      Medicine, Roswell Park Cancer Institute, Buffalo, New York 14263, USA. 
      hans.minderman@roswellpark.org
FAU - Humphrey, Kristen
AU  - Humphrey K
FAU - Arcadi, Jane K
AU  - Arcadi JK
FAU - Wierzbicki, Andrzej
AU  - Wierzbicki A
FAU - Maguire, Orla
AU  - Maguire O
FAU - Wang, Eunice S
AU  - Wang ES
FAU - Block, AnneMarie W
AU  - Block AW
FAU - Sait, Sheila N J
AU  - Sait SN
FAU - George, Thaddeus C
AU  - George TC
FAU - Wallace, Paul K
AU  - Wallace PK
LA  - eng
GR  - S10 RR022335/RR/NCRR NIH HHS/United States
GR  - P30 CA016056/CA/NCI NIH HHS/United States
GR  - 4R33CA126667/CA/NCI NIH HHS/United States
GR  - 1S10RR022335-01/RR/NCRR NIH HHS/United States
GR  - CA016056/CA/NCI NIH HHS/United States
GR  - R33 CA126667/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20120726
PL  - United States
TA  - Cytometry A
JT  - Cytometry. Part A : the journal of the International Society for Analytical 
      Cytology
JID - 101235694
SB  - IM
MH  - Algorithms
MH  - *Aneuploidy
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - Image Processing, Computer-Assisted
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia, Myeloid, Acute/genetics/*pathology
MH  - Leukocytes, Mononuclear/*pathology
MH  - Limit of Detection
MH  - Male
MH  - Models, Biological
MH  - Reproducibility of Results
MH  - Single-Cell Analysis
PMC - PMC4384178
MID - NIHMS667497
EDAT- 2012/07/28 06:00
MHDA- 2012/12/27 06:00
PMCR- 2015/04/03
CRDT- 2012/07/28 06:00
PHST- 2012/04/02 00:00 [received]
PHST- 2012/06/04 00:00 [revised]
PHST- 2012/06/20 00:00 [accepted]
PHST- 2012/07/28 06:00 [entrez]
PHST- 2012/07/28 06:00 [pubmed]
PHST- 2012/12/27 06:00 [medline]
PHST- 2015/04/03 00:00 [pmc-release]
AID - 10.1002/cyto.a.22101 [doi]
PST - ppublish
SO  - Cytometry A. 2012 Sep;81(9):776-84. doi: 10.1002/cyto.a.22101. Epub 2012 Jul 26.