PMID- 22840825
OWN - NLM
STAT- MEDLINE
DCOM- 20121113
LR  - 20151119
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1253
DP  - 2012 Aug 31
TI  - Analysis of phenolic pollutants in human samples by high performance capillary 
      electrophoresis based on pretreatment of ultrasound-assisted emulsification 
      microextraction and solidification of floating organic droplet.
PG  - 16-21
LID - 10.1016/j.chroma.2012.06.088 [doi]
AB  - A novel, simple and rapid method, termed ultrasound-assisted emulsification 
      microextraction and solidification of floating organic droplet (UAEM-SFO) coupled 
      to high performance capillary electrophoresis (HPCE), was developed for 
      preconcentration and analysis of five phenolic compounds (PCs) in human urine and 
      blood samples. The proposed method is based on microextraction of target analytes 
      in less-toxic organic solvent under assistance of ultrasound. A micro-droplet of 
      less-toxic organic solvent floating on the surface of liquid samples in a sealed 
      vial can be dispersed into sample solutions under the ultrasound frequency, 
      solidified under ice bath, collected with a medicine spatula, molten at ambient 
      temperature, and finally subjected to HPCE analysis. The parameters of UAEM-SFO 
      procedure including type and volume of extraction solvent, extraction 
      temperature, time of ultrasound-assisted extraction and centrifugation, sample 
      pH, and ionic strength were optimized. The influence parameters on HPCE 
      resolution such as pH, concentration of running buffer, applied voltage and 
      injection time were also investigated. This method requires only 40 muL of 
      2-dodecanol extraction solvent and 8 min of pretreatment time. The enrichment 
      factors of analytes were in the range of 114-172 and extraction recoveries 
      (69-86%) were obtained. Good linearity was achieved for five analytes in the 
      range of 0.05-100 mug L(-)(1) and the correlation coefficients ranged from 0.9934 to 
      0.9999. The limits of detection were 0.01 mug L(-)(1) for triclosan (TCS) and 
      biophenol A (BPA), and 0.02 mug L(-)(1) for pentachlorophenol (PCP), 
      2,4-dichlorophenol (2,4-DCP) and 4-nonylphenol (4-NP) in urine samples, and 0.02 
      mug L(-)(1) for TCS and BPA, 0.04 mug L(-)(1) for PCP, 2,4-DCP and 4-NP in blood samples. 
      The developed UAEM-SFO-HPCE method has a great potential in routine residual 
      analysis of trace PCs in biological samples.
CI  - Copyright (c) 2012. Published by Elsevier B.V.
FAU - Wang, Huili
AU  - Wang H
AD  - School of Life Sciences, Wenzhou Medical College, University-Town, Wenzhou, 
      Zhejiang 325035, China.
FAU - Yan, Hao
AU  - Yan H
FAU - Wang, Chengjun
AU  - Wang C
FAU - Chen, Fan
AU  - Chen F
FAU - Ma, Meiping
AU  - Ma M
FAU - Wang, Wenwei
AU  - Wang W
FAU - Wang, Xuedong
AU  - Wang X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120704
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Emulsions)
RN  - 0 (Phenols)
RN  - 451W47IQ8X (Sodium Chloride)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Centrifugation
MH  - Chemical Fractionation/*methods
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Electrophoresis, Capillary/*methods
MH  - Emulsions
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Limit of Detection
MH  - Occupational Exposure
MH  - Phenols/*blood/chemistry/isolation & purification/*urine
MH  - Reproducibility of Results
MH  - Sodium Chloride/chemistry
MH  - Sonication/*methods
MH  - Temperature
EDAT- 2012/07/31 06:00
MHDA- 2012/11/14 06:00
CRDT- 2012/07/31 06:00
PHST- 2012/03/06 00:00 [received]
PHST- 2012/06/27 00:00 [revised]
PHST- 2012/06/29 00:00 [accepted]
PHST- 2012/07/31 06:00 [entrez]
PHST- 2012/07/31 06:00 [pubmed]
PHST- 2012/11/14 06:00 [medline]
AID - S0021-9673(12)01008-4 [pii]
AID - 10.1016/j.chroma.2012.06.088 [doi]
PST - ppublish
SO  - J Chromatogr A. 2012 Aug 31;1253:16-21. doi: 10.1016/j.chroma.2012.06.088. Epub 
      2012 Jul 4.