PMID- 22844266
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220331
IS  - 1616-301X (Print)
IS  - 1616-3028 (Electronic)
IS  - 1616-301X (Linking)
VI  - 21
IP  - 22
DP  - 2011 Nov 22
TI  - Multifunctionalized electrospun silk fibers promote axon regeneration in central 
      nervous system.
PG  - 4202
AB  - The repair of central nerves remains a major challenge in regenerative 
      neurobiology. Regenerative guides possessing critical features such as cell 
      adhesion, physical guiding and topical stimulation are needed. To generate such a 
      guide, silk protein materials are prepared using electrospinning. The silk is 
      selected for this study due to its biocompatibility and ability to be electrospun 
      for the formation of aligned biofunctional nanofibers. The addition of Brain 
      Derived Neurotrophic Factor (BDNF), Ciliary Neurotrophic Factor (CNTF) or both to 
      the electrospun fibers enable enhanced function without impact to the structure 
      or the surface morphology. Only a small fraction of the loaded growth factors is 
      released over time allowing the fibers to continue to provide these factors to 
      the cells for extended periods of time. The entrapped factors remain active and 
      available to the cells as rat retinal ganglion cells (RGCs) exhibit longer axonal 
      growth when in contact with the biofunctionalized fibers. Compare to 
      non-functionalized fibers, the growth of neurites increased 2 fold on fibers 
      containing BDNF, 2.5 fold with fibers containing CNTF and by almost 3-fold on 
      fibers containing both factors. The results demonstrate the potential of aligned 
      and functionalized electrospun silk fibers to promote nerve growth in the central 
      nervous system, underlying the great potential of complex biomaterials in 
      neuroregenerative strategies following axotomy and nerve crush traumas.
FAU - Wittmer, Corinne R
AU  - Wittmer CR
AD  - Department of Biomedical Engineering, Tufts University, 4 Colby Street, Medford, 
      MA 02155 (USA).
FAU - Claudepierre, Thomas
AU  - Claudepierre T
FAU - Reber, Michael
AU  - Reber M
FAU - Wiedemann, Peter
AU  - Wiedemann P
FAU - Garlick, Jonathan A
AU  - Garlick JA
FAU - Kaplan, David
AU  - Kaplan D
FAU - Egles, Christophe
AU  - Egles C
LA  - eng
GR  - P41 EB002520/EB/NIBIB NIH HHS/United States
GR  - R01 AR055993/AR/NIAMS NIH HHS/United States
GR  - R01 AR061988/AR/NIAMS NIH HHS/United States
PT  - Journal Article
DEP - 20111116
PL  - Germany
TA  - Adv Funct Mater
JT  - Advanced functional materials
JID - 101190390
PMC - PMC3404853
MID - NIHMS389677
EDAT- 2012/07/31 06:00
MHDA- 2012/07/31 06:01
PMCR- 2012/11/22
CRDT- 2012/07/31 06:00
PHST- 2012/07/31 06:00 [entrez]
PHST- 2012/07/31 06:00 [pubmed]
PHST- 2012/07/31 06:01 [medline]
PHST- 2012/11/22 00:00 [pmc-release]
AID - 10.1002/adfm.201190103 [doi]
PST - ppublish
SO  - Adv Funct Mater. 2011 Nov 22;21(22):4202. doi: 10.1002/adfm.201190103. Epub 2011 
      Nov 16.