PMID- 22844318
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20211021
IS  - 2092-7258 (Electronic)
IS  - 1738-1061 (Print)
IS  - 1738-1061 (Linking)
VI  - 55
IP  - 7
DP  - 2012 Jul
TI  - Neuroprotective effects of L-carnitine against oxygen-glucose deprivation in rat 
      primary cortical neurons.
PG  - 238-48
LID - 10.3345/kjp.2012.55.7.238 [doi]
AB  - PURPOSE: Hypoxic-ischemic encephalopathy is an important cause of neonatal 
      mortality, as this brain injury disrupts normal mitochondrial respiratory 
      activity. Carnitine plays an essential role in mitochondrial fatty acid transport 
      and modulates excess acyl coenzyme A levels. In this study, we investigated 
      whether treatment of primary cultures of rat cortical neurons with L-carnitine 
      was able to prevent neurotoxicity resulting from oxygen-glucose deprivation 
      (OGD). METHODS: Cortical neurons were prepared from Sprague-Dawley rat embryos. 
      L-Carnitine was applied to cultures just prior to OGD and subsequent 
      reoxygenation. The numbers of cells that stained with acridine orange (AO) and 
      propidium iodide (PI) were counted, and lactate dehydrogenase (LDH) activity and 
      reactive oxygen species (ROS) levels were measured. The 
      3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and the 
      terminal uridine deoxynucleotidyl transferase-mediated deoxyuridine triphosphate 
      nick-end labeling assay were performed to evaluate the effect of L-carnitine (1 
      microM, 10 microM, and 100 microM) on OGD-induced neurotoxicity. RESULTS: Treatment of 
      primary cultures of rat cortical neurons with L-carnitine significantly reduced 
      cell necrosis and prevented apoptosis after OGD. L-Carnitine application 
      significantly reduced the number of cells that died, as assessed by the PI/AO 
      ratio, and also reduced ROS release in the OGD groups treated with 10 microM and 100 
      microM of L-carnitine compared with the untreated OGD group (P<0.05). The application 
      of L-carnitine at 100 microM significantly decreased cytotoxicity, LDH release, and 
      inhibited apoptosis compared to the untreated OGD group (P<0.05). CONCLUSION: 
      L-Carnitine has neuroprotective benefits against OGD in rat primary cortical 
      neurons in vitro.
FAU - Kim, Yu Jin
AU  - Kim YJ
AD  - Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School 
      of Medicine, Seoul, Korea.
FAU - Kim, Soo Yoon
AU  - Kim SY
FAU - Sung, Dong Kyung
AU  - Sung DK
FAU - Chang, Yun Sil
AU  - Chang YS
FAU - Park, Won Soon
AU  - Park WS
LA  - eng
PT  - Journal Article
DEP - 20120717
PL  - Korea (South)
TA  - Korean J Pediatr
JT  - Korean journal of pediatrics
JID - 101215374
PMC - PMC3405156
OTO - NOTNLM
OT  - Hypoxia-Ischemia
OT  - L-carnitine
OT  - Neurons
OT  - Neuroprotective effect
OT  - Oxygen-glucose deprivation
EDAT- 2012/07/31 06:00
MHDA- 2012/07/31 06:01
PMCR- 2012/07/01
CRDT- 2012/07/31 06:00
PHST- 2011/08/01 00:00 [received]
PHST- 2012/02/15 00:00 [revised]
PHST- 2012/03/20 00:00 [accepted]
PHST- 2012/07/31 06:00 [entrez]
PHST- 2012/07/31 06:00 [pubmed]
PHST- 2012/07/31 06:01 [medline]
PHST- 2012/07/01 00:00 [pmc-release]
AID - 10.3345/kjp.2012.55.7.238 [doi]
PST - ppublish
SO  - Korean J Pediatr. 2012 Jul;55(7):238-48. doi: 10.3345/kjp.2012.55.7.238. Epub 
      2012 Jul 17.