PMID- 22846543
OWN - NLM
STAT- MEDLINE
DCOM- 20130109
LR  - 20220409
IS  - 1873-3913 (Electronic)
IS  - 0898-6568 (Linking)
VI  - 24
IP  - 11
DP  - 2012 Nov
TI  - Wogonin induces apoptosis by activating the AMPK and p53 signaling pathways in 
      human glioblastoma cells.
PG  - 2216-25
LID - 10.1016/j.cellsig.2012.07.019 [doi]
AB  - We investigated the molecular basis of the ability of wogonin to control the 
      intracellular signaling cascades of AMP-activated protein kinase (AMPK). This 
      activity induces antitumor activities in glioblastoma multiforme (GBM) cells. 
      Recently, the evolutionarily conserved serine/threonine kinase AMPK has emerged 
      as a possible target for tumor control. We investigated the effects of wogonin on 
      apoptosis regulation and the activation of AMPK. Wogonin treatment resulted in a 
      series of antitumor effects such as cell death and apoptotic appearance. 
      Activation of AMPK suppressed downstream substrates, such as the mammalian target 
      of rapamycin (mTOR) and eukaryotic initiation factor 4E-binding protein-1 
      (4E-BP1), and resulted in a general decrease in translation. Moreover, 
      wogonin-activated AMPK decreased the activity and/or expression of lipogenic 
      enzymes such as acetyl-CoA carboxylase. Furthermore, in GBM cells, wogonin 
      blocked cell cycle progression at the G1 phase and induced apoptosis by inducing 
      p53 expression and further upregulating p21 expression. Taken together, our 
      findings demonstrated that wogonin has the potential to be a chemopreventive and 
      therapeutic agent against human GBM.
CI  - Copyright (c) 2012 Elsevier Inc. All rights reserved.
FAU - Lee, Dae-Hee
AU  - Lee DH
AD  - Department of Neurosurgery, University of Virginia, School of Medicine, VA, USA.
FAU - Lee, Tae Hwa
AU  - Lee TH
FAU - Jung, Chang Hwa
AU  - Jung CH
FAU - Kim, Young-Ho
AU  - Kim YH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120728
PL  - England
TA  - Cell Signal
JT  - Cellular signalling
JID - 8904683
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (EIF4EBP1 protein, human)
RN  - 0 (Flavanones)
RN  - 0 (Phosphoproteins)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
RN  - EC 3.4.22.- (Caspases)
RN  - EC 6.4.1.2 (Acetyl-CoA Carboxylase)
RN  - POK93PO28W (wogonin)
SB  - IM
MH  - AMP-Activated Protein Kinases/*metabolism
MH  - Acetyl-CoA Carboxylase/metabolism
MH  - Adaptor Proteins, Signal Transducing/metabolism
MH  - Apoptosis/*drug effects
MH  - Caspases/metabolism
MH  - Cell Cycle Proteins
MH  - Cell Line, Tumor
MH  - DNA Damage/drug effects
MH  - Drugs, Chinese Herbal
MH  - Flavanones/*pharmacology
MH  - Glioblastoma/metabolism/pathology
MH  - Humans
MH  - Phosphoproteins/metabolism
MH  - Reactive Oxygen Species/metabolism
MH  - Signal Transduction/*drug effects
MH  - TOR Serine-Threonine Kinases/metabolism
MH  - Tumor Suppressor Protein p53/*metabolism
EDAT- 2012/08/01 06:00
MHDA- 2013/01/10 06:00
CRDT- 2012/08/01 06:00
PHST- 2012/05/18 00:00 [received]
PHST- 2012/07/10 00:00 [revised]
PHST- 2012/07/24 00:00 [accepted]
PHST- 2012/08/01 06:00 [entrez]
PHST- 2012/08/01 06:00 [pubmed]
PHST- 2013/01/10 06:00 [medline]
AID - S0898-6568(12)00215-X [pii]
AID - 10.1016/j.cellsig.2012.07.019 [doi]
PST - ppublish
SO  - Cell Signal. 2012 Nov;24(11):2216-25. doi: 10.1016/j.cellsig.2012.07.019. Epub 
      2012 Jul 28.