PMID- 22847014
OWN - NLM
STAT- MEDLINE
DCOM- 20130111
LR  - 20220316
IS  - 1768-3238 (Electronic)
IS  - 1631-0691 (Linking)
VI  - 335
IP  - 7
DP  - 2012 Jul
TI  - Identification of ancient Olea europaea L. and Cornus mas L. seeds by DNA 
      barcoding.
PG  - 472-9
LID - 10.1016/j.crvi.2012.05.004 [doi]
AB  - The analysis of ancient DNA (aDNA) provides archaeologists and anthropologists 
      with innovative, scientific and accurate data to study and understand the past. 
      In this work, ancient seeds, found in the "Mora Cavorso" archaeological site 
      (Latium, Central Italy), were analyzed to increase information about Italian 
      Neolithic populations (plant use, agriculture, diet, trades, customs and 
      ecology). We performed morphological and genetic techniques to identify fossil 
      botanical species. In particular, this study also suggests and emphasizes the use 
      of DNA barcode method for ancient plant sample analysis. Scanning electron 
      microscope (SEM) observations showed seed compact structure and irregular surface 
      but they did not permit a precise nor empirical classification: so, a molecular 
      approach was necessary. DNA was extracted from ancient seeds and then it was 
      used, as template, for PCR amplifications of standardized barcode genes. Although 
      aDNA could be highly degraded by the time, successful PCR products were obtained, 
      sequenced and compared to nucleotide sequence databases. Positive outcomes 
      (supported by morphological comparison with modern seeds, geographical 
      distribution and historical data) indicated that seeds could be identified as 
      belonging to two plant species: Olea europaea L. and Cornus mas L.
CI  - Copyright (c) 2012 Academie des sciences. Published by Elsevier SAS. All rights 
      reserved.
FAU - Gismondi, Angelo
AU  - Gismondi A
AD  - Department of biology, university of Rome "Tor Vergata", Italy. ang84@hotmail.it
FAU - Rolfo, Mario Federico
AU  - Rolfo MF
FAU - Leonardi, Donatella
AU  - Leonardi D
FAU - Rickards, Olga
AU  - Rickards O
FAU - Canini, Antonella
AU  - Canini A
LA  - eng
PT  - Historical Article
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120629
PL  - France
TA  - C R Biol
JT  - Comptes rendus biologies
JID - 101140040
RN  - 0 (DNA, Plant)
SB  - IM
MH  - Agriculture/*history
MH  - Archaeology/*methods
MH  - Caves
MH  - Cornus/classification/*genetics
MH  - DNA Barcoding, Taxonomic
MH  - DNA, Plant/*analysis/isolation & purification
MH  - Databases, Genetic
MH  - *Fossils
MH  - *Genes, Plant
MH  - History, Ancient
MH  - Italy
MH  - Microscopy, Electron, Scanning
MH  - Olea/classification/*genetics
MH  - Polymerase Chain Reaction/methods
MH  - Seeds/*chemistry/ultrastructure
MH  - Sequence Alignment
MH  - Sequence Homology, Nucleic Acid
MH  - Species Specificity
EDAT- 2012/08/01 06:00
MHDA- 2013/01/12 06:00
CRDT- 2012/08/01 06:00
PHST- 2012/04/23 00:00 [received]
PHST- 2012/05/17 00:00 [revised]
PHST- 2012/05/18 00:00 [accepted]
PHST- 2012/08/01 06:00 [entrez]
PHST- 2012/08/01 06:00 [pubmed]
PHST- 2013/01/12 06:00 [medline]
AID - S1631-0691(12)00135-7 [pii]
AID - 10.1016/j.crvi.2012.05.004 [doi]
PST - ppublish
SO  - C R Biol. 2012 Jul;335(7):472-9. doi: 10.1016/j.crvi.2012.05.004. Epub 2012 Jun 
      29.