PMID- 22847550
OWN - NLM
STAT- MEDLINE
DCOM- 20130913
LR  - 20211021
IS  - 1573-6830 (Electronic)
IS  - 0272-4340 (Linking)
VI  - 32
IP  - 8
DP  - 2012 Nov
TI  - BDNF-dependent accumulation of palmitoleic acid in CNS neurons.
PG  - 1367-73
LID - 10.1007/s10571-012-9863-x [doi]
AB  - Although it is known that brain-derived neurotrophic factor (BDNF) plays a 
      critical role in neuronal survival and differentiation, its effect on lipid 
      homeostasis is poorly understood. To understand them, we here investigated the 
      effect of BDNF on the fatty acid composition of primary neurons. A detailed 
      analysis of the fatty acid composition of BDNF-stimulated primary neurons 
      revealed that BDNF treatment led to a significant and selective increase in 
      intracellular palmitoleic acid (PLO) levels. Correspondingly, BDNF induced the 
      expression of the enzyme responsible for PLO synthesis [stearoyl-CoA 
      desaturase-1]. In addition, this increase was suppressed by K252a, an inhibitor 
      for tropomyosin-related kinase (Trk) receptors, indicating that BDNF-dependent 
      increase in the PLO was mediated through the activation of TrkB. Further, PLO in 
      culture media was reduced by BDNF treatment. This result suggested that BDNF 
      suppressed extracellular release of PLO. Taken together, these data indicate that 
      BDNF increases intracellular PLO both by activating its biosynthesis and by 
      suppressing its extracellular release.
FAU - Suzuki, Shingo
AU  - Suzuki S
AD  - Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University, 
      1750-1 Ikenobe, Miki-cho, Kagawa, 761-0793, Japan. ssuzuki@med.kagawa-u.ac.jp
FAU - Hongli, Qiu
AU  - Hongli Q
FAU - Okada, Aya
AU  - Okada A
FAU - Kasama, Takeshi
AU  - Kasama T
FAU - Ohta, Ken-Ichi
AU  - Ohta K
FAU - Warita, Katsuhiko
AU  - Warita K
FAU - Tanaka, Kohichi
AU  - Tanaka K
FAU - Miki, Takanori
AU  - Miki T
FAU - Takeuchi, Yoshiki
AU  - Takeuchi Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120731
PL  - United States
TA  - Cell Mol Neurobiol
JT  - Cellular and molecular neurobiology
JID - 8200709
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Fatty Acids, Monounsaturated)
RN  - 209B6YPZ4I (palmitoleic acid)
RN  - EC 1.14.19.1 (Stearoyl-CoA Desaturase)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*physiology
MH  - Cells, Cultured
MH  - Central Nervous System/cytology/enzymology/*metabolism
MH  - Cerebral Cortex/cytology/enzymology/*metabolism
MH  - Fatty Acids, Monounsaturated/*metabolism
MH  - Hippocampus/cytology/enzymology/metabolism
MH  - Intracellular Space/enzymology/metabolism
MH  - Neurons/enzymology/*metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Stearoyl-CoA Desaturase/biosynthesis
MH  - Tissue Distribution/physiology
EDAT- 2012/08/01 06:00
MHDA- 2013/09/14 06:00
CRDT- 2012/08/01 06:00
PHST- 2012/05/21 00:00 [received]
PHST- 2012/07/14 00:00 [accepted]
PHST- 2012/08/01 06:00 [entrez]
PHST- 2012/08/01 06:00 [pubmed]
PHST- 2013/09/14 06:00 [medline]
AID - 10.1007/s10571-012-9863-x [doi]
PST - ppublish
SO  - Cell Mol Neurobiol. 2012 Nov;32(8):1367-73. doi: 10.1007/s10571-012-9863-x. Epub 
      2012 Jul 31.