PMID- 22847693
OWN - NLM
STAT- MEDLINE
DCOM- 20121129
LR  - 20120731
IS  - 1097-0231 (Electronic)
IS  - 0951-4198 (Linking)
VI  - 26
IP  - 17
DP  - 2012 Sep 15
TI  - Metabolism profile of timosaponin B-II in urine after oral administration to rats 
      by ultrahigh-performance liquid chromatography/quadrupole-time-of-flight mass 
      spectrometry.
PG  - 1955-64
LID - 10.1002/rcm.6299 [doi]
AB  - RATIONALE: Timosaponin B-II (TB-II) is one of the major bioactive steroid 
      glycosides isolated from Anemarrhena asphodeloides Bge. (Fam. Liliaceae). It has 
      been regarded as a potential lead compound, which may be further developed into a 
      promising new drug for preventing dementia. To fully understand the action 
      mechanism of TB-II, it is important to study the metabolism profile of this 
      compound in vivo. METHODS: Herein, a rapid and sensitive method based on 
      ultrahigh-performance liquid chromatography (UHPLC)/quadrupole-time-of-flight 
      mass spectrometry (QTOFMS) was established to comprehensively investigate the 
      metabolism of TB-II in Sprague-Dawley rat urine following oral administration of 
      a single dose of TB-II at 500.4 mg.kg(-1). RESULTS: A total of twelve metabolites 
      were detected and identified by means of comparing molecular mass, retention time 
      and spectral pattern of the analytes with those of the parent drug. A possible 
      metabolic pathway on the biotransformation of TB-II was also investigated and 
      proposed. CONCLUSIONS: Oxidation, deglycosylation and E-ring cleavage were found 
      to be the major metabolic processes of the compound in rat. It is the first 
      report on a mammalian metabolism study of timosaponin, a common member of steroid 
      glycosides, in rat urine.
CI  - Copyright (c) 2012 John Wiley & Sons, Ltd.
FAU - Liu, Zhirui
AU  - Liu Z
AD  - School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
FAU - Zhu, Dongliang
AU  - Zhu D
FAU - Lv, Lei
AU  - Lv L
FAU - Li, Yueyue
AU  - Li Y
FAU - Dong, Xing
AU  - Dong X
FAU - Zhu, Zhenyu
AU  - Zhu Z
FAU - Chai, Yifeng
AU  - Chai Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Rapid Commun Mass Spectrom
JT  - Rapid communications in mass spectrometry : RCM
JID - 8802365
RN  - 0 (Saponins)
RN  - 0 (Steroids)
RN  - 0 (timosaponin B-II)
SB  - IM
MH  - Administration, Oral
MH  - Animals
MH  - Biotransformation
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Male
MH  - Mass Spectrometry/*methods
MH  - Metabolic Networks and Pathways
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Saponins/administration & dosage/chemistry/*pharmacokinetics/*urine
MH  - Sensitivity and Specificity
MH  - Steroids/administration & dosage/chemistry/*pharmacokinetics/*urine
EDAT- 2012/08/01 06:00
MHDA- 2012/12/10 06:00
CRDT- 2012/08/01 06:00
PHST- 2012/08/01 06:00 [entrez]
PHST- 2012/08/01 06:00 [pubmed]
PHST- 2012/12/10 06:00 [medline]
AID - 10.1002/rcm.6299 [doi]
PST - ppublish
SO  - Rapid Commun Mass Spectrom. 2012 Sep 15;26(17):1955-64. doi: 10.1002/rcm.6299.