PMID- 22848163
OWN - NLM
STAT- MEDLINE
DCOM- 20130221
LR  - 20211021
IS  - 1178-2013 (Electronic)
IS  - 1176-9114 (Print)
IS  - 1176-9114 (Linking)
VI  - 7
DP  - 2012
TI  - Engineered hepatitis B virus surface antigen L protein particles for in vivo 
      active targeting of splenic dendritic cells.
PG  - 3341-50
LID - 10.2147/IJN.S32813 [doi]
AB  - Dendritic cells (DCs) are key regulators of adaptive T-cell responses. By 
      capturing exogenous antigens and presenting antigen-derived peptides via major 
      histocompatibility complex molecules to naive T cells, DCs induce 
      antigen-specific immune responses in vivo. In order to induce effective host 
      immune responses, active delivery of exogenous antigens to DCs is considered 
      important for future vaccine development. We recently generated bionanocapsules 
      (BNCs) consisting of hepatitis B virus surface antigens that mediate stringent in 
      vivo cell targeting and efficient endosomal escape, and after the fusion with 
      liposomes (LP) containing therapeutic materials, the BNC-LP complexes deliver 
      them to human liver-derived tissues in vivo. BNCs were further modified to 
      present the immunoglobulin G (IgG) Fc-interacting domain (Z domain) derived from 
      Staphylococcus aureus protein A in tandem. When mixed with IgGs, modified BNCs 
      (ZZ-BNCs) displayed the IgG Fv regions outwardly for efficient binding to 
      antigens in an oriented-immobilization manner. Due to the affinity of the 
      displayed IgGs, the IgG-ZZ-BNC complexes accumulated in specific cells and 
      tissues in vitro and in vivo. After mixing ZZ-BNCs with antibodies against DCs, 
      we used immunocytochemistry to examine which antibodies delivered ZZ-BNCs to 
      mouse splenic DCs following intravenous injection of the ZZ-BNCs. ZZ-BNCs 
      displaying anti-CD11c monoclonal antibodies (alpha-CD11c-ZZ-BNCs) were found to 
      accumulate with approximately 62% of splenic DCs, and reside within some of them. 
      After the fusion with liposomes containing antigens, the alpha-CD11c-ZZ-BNCs could 
      elicit the respective antibodies more efficiently than other nontargeting control 
      vaccines, suggesting that this DC-specific nanocarrier is promising for future 
      vaccines.
FAU - Matsuo, Hidenori
AU  - Matsuo H
AD  - Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan.
FAU - Yoshimoto, Nobuo
AU  - Yoshimoto N
FAU - Iijima, Masumi
AU  - Iijima M
FAU - Niimi, Tomoaki
AU  - Niimi T
FAU - Jung, Joohee
AU  - Jung J
FAU - Jeong, Seong-Yun
AU  - Jeong SY
FAU - Choi, Eun Kyung
AU  - Choi EK
FAU - Sewaki, Tomomitsu
AU  - Sewaki T
FAU - Arakawa, Takeshi
AU  - Arakawa T
FAU - Kuroda, Shun'ichi
AU  - Kuroda S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120703
PL  - New Zealand
TA  - Int J Nanomedicine
JT  - International journal of nanomedicine
JID - 101263847
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, Viral)
RN  - 0 (Bacterial Vaccines)
RN  - 0 (Hepatitis B Surface Antigens)
RN  - 0 (L protein, hepatitis B virus)
RN  - 0 (Liposomes)
RN  - 0 (Nanocapsules)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Staphylococcal Protein A)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/metabolism
MH  - Antigens, Viral/immunology
MH  - Bacterial Vaccines/chemistry/immunology/metabolism/pharmacokinetics
MH  - Dendritic Cells/*metabolism
MH  - Encephalitis Virus, Japanese/immunology
MH  - Female
MH  - Hepatitis B Surface Antigens/chemistry/*metabolism
MH  - Liposomes/chemistry/immunology/metabolism/pharmacokinetics
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Nanocapsules/administration & dosage/*chemistry
MH  - Recombinant Fusion Proteins/chemistry/immunology/*metabolism/pharmacokinetics
MH  - Spleen/cytology
MH  - Staphylococcal Protein A/chemistry/genetics/metabolism
MH  - Tissue Distribution
MH  - Viral Envelope Proteins/chemistry/*metabolism
MH  - Viral Proteins/immunology
PMC - PMC3405891
OTO - NOTNLM
OT  - ZZ domain
OT  - drug-delivery system
OT  - gene-delivery system
OT  - liposomes
OT  - protein A
OT  - vaccine
EDAT- 2012/08/01 06:00
MHDA- 2013/02/22 06:00
PMCR- 2012/07/03
CRDT- 2012/08/01 06:00
PHST- 2012/08/01 06:00 [entrez]
PHST- 2012/08/01 06:00 [pubmed]
PHST- 2013/02/22 06:00 [medline]
PHST- 2012/07/03 00:00 [pmc-release]
AID - ijn-7-3341 [pii]
AID - 10.2147/IJN.S32813 [doi]
PST - ppublish
SO  - Int J Nanomedicine. 2012;7:3341-50. doi: 10.2147/IJN.S32813. Epub 2012 Jul 3.