PMID- 22849299
OWN - NLM
STAT- MEDLINE
DCOM- 20130124
LR  - 20211021
IS  - 1476-511X (Electronic)
IS  - 1476-511X (Linking)
VI  - 11
DP  - 2012 Jul 31
TI  - Mononuclear cell therapy reverts cuff-induced thrombosis in apolipoprotein 
      E-deficient mice.
PG  - 96
LID - 10.1186/1476-511X-11-96 [doi]
AB  - BACKGROUND: Stem/progenitor cell-based therapy has successfully been used as a 
      novel therapeutic strategy for vascular diseases triggered by endothelial 
      dysfunction. The aim of this study was to investigate the effects of mononuclear 
      cell (MNC) therapy in situ on carotid cuff-induced occlusive thrombus in the 
      apolipoprotein E knockout (apoE-/-) mouse. METHODS: Spleen-derived MNCs were 
      isolated from green fluorescent protein (GFP)-transgenic mice for cell treatment. 
      A cuff-induced thrombus model was produced by placing a nonconstrictive silastic 
      collar around the left common carotid artery in 20-week-old female apoE-/- mice. 
      After 10 days, the cuff was removed, and the animals received in situ MNCs 
      (Cuff-MNC) or vehicle (Cuff-Vehicle) and were compared with sham-operated animals 
      (Sham). RESULTS: The histological analysis showed that the MNC treatment reverted 
      occlusive thrombus formation compared to the vehicle and the vessel lumen area to 
      that observed in the Sham group (MNC, 50 +/- 4; Vehicle, 20 +/- 4; Sham, 55 +/- 2 x10(3) 
      mum(2); p < 0.01). The animals that underwent the carotid cuff placement developed 
      compensatory vessel enlargement, which was reduced by the MNC therapy. In 
      addition, the treatment was able to reduce superoxide anion production, which 
      likely contributed to the reduced apoptosis that was observed. Lastly, the 
      immunofluorescence analysis revealed the presence of endothelial progenitor cells 
      (EPCs) in the carotid endothelia of the apoE-/- mice. CONCLUSION: In situ 
      short-term MNC therapy was able to revert cuff-induced occlusive thrombi in the 
      carotid arteries of apoE-/- mice, possibly through the homing of EPCs, reduction 
      of oxidative stress and decreased apoptosis.
FAU - Lima, Leandro C F
AU  - Lima LC
AD  - Laboratory of Transgenes and Cardiovascular Control, Department Physiological 
      Sciences, Health Sciences Center, Federal University of Espirito Santo, Vitoria, 
      ES, Brazil.
FAU - Porto, Marcella L
AU  - Porto ML
FAU - Campagnaro, Bianca P
AU  - Campagnaro BP
FAU - Tonini, Clarissa L
AU  - Tonini CL
FAU - Nogueira, Breno V
AU  - Nogueira BV
FAU - Pereira, Thiago Mc
AU  - Pereira TM
FAU - Vasquez, Elisardo C
AU  - Vasquez EC
FAU - Meyrelles, Silvana S
AU  - Meyrelles SS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120731
PL  - England
TA  - Lipids Health Dis
JT  - Lipids in health and disease
JID - 101147696
RN  - 0 (Apolipoproteins E)
RN  - 0 (Recombinant Proteins)
RN  - 11062-77-4 (Superoxides)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Apolipoproteins E/*deficiency/genetics
MH  - Apoptosis
MH  - Atherosclerosis/metabolism/pathology
MH  - Carotid Artery, Common/pathology
MH  - Endothelial Cells/pathology
MH  - Female
MH  - Green Fluorescent Proteins/biosynthesis
MH  - Leukocytes, Mononuclear/*transplantation
MH  - Ligation
MH  - Mice
MH  - Mice, Knockout
MH  - Microscopy, Fluorescence
MH  - Oxidative Stress
MH  - Recombinant Proteins/biosynthesis
MH  - Superoxides/metabolism
MH  - Thrombosis/etiology/*therapy
PMC - PMC3477089
EDAT- 2012/08/02 06:00
MHDA- 2013/01/25 06:00
PMCR- 2012/07/31
CRDT- 2012/08/02 06:00
PHST- 2012/06/08 00:00 [received]
PHST- 2012/07/12 00:00 [accepted]
PHST- 2012/08/02 06:00 [entrez]
PHST- 2012/08/02 06:00 [pubmed]
PHST- 2013/01/25 06:00 [medline]
PHST- 2012/07/31 00:00 [pmc-release]
AID - 1476-511X-11-96 [pii]
AID - 10.1186/1476-511X-11-96 [doi]
PST - epublish
SO  - Lipids Health Dis. 2012 Jul 31;11:96. doi: 10.1186/1476-511X-11-96.