PMID- 22849519
OWN - NLM
STAT- MEDLINE
DCOM- 20130301
LR  - 20131121
IS  - 1744-5116 (Electronic)
IS  - 1388-0209 (Linking)
VI  - 50
IP  - 11
DP  - 2012 Nov
TI  - Anticancer activities of the extract from Longkong (Lansium domesticum) young 
      fruits.
PG  - 1397-407
LID - 10.3109/13880209.2012.682116 [doi]
AB  - "Longkong"(Lansium domesticum Corr., Family: Meliaceae) is a fruit found in the 
      south of Thailand. This plant has been used in traditional medicines. OBJECTIVES: 
      To investigate the antiproliferative activities and the phytoconstituents of 
      Longkong extracts. MATERIALS AND METHODS: Cytotoxicity and apoptotic activity of 
      48 extracts were tested using the SRB assay and acridine orange (AO)/ethidium 
      bromide (EB) staining, respectively. The extracts which gave the highest 
      anticancer activity were selected to prepare the semipurified extracts and 
      analysis for the constituents by gas chromatography-mass spectrometry (GC/MS). 
      RESULTS: The highest percentage yield (59.38%) was from the cold water extract of 
      Longkong ripe fruits (RFWC). The highest total phenolic and flavonoid contents 
      were observed in cold and hot methanol extract of Longkong stalks (STMC and 
      STMH). The hot and cold chloroform young Longkong fruit extracts (YFCH and YFCC) 
      exhibited a cytotoxic effect (IC(50) < 1 mg/mL) against cancer cells. For 
      apoptotic induction, YFCH demonstrated the highest activity against KB of 13.84 +/- 
      4.21% at 0.5 mg/mL which was 0.88 and 1.35 times of cisplatin and 5-FU, 
      respectively, while apoptotic cells in HT-29 were 8.68 +/- 1.85% at 5 mg/mL, which 
      was 0.61 and 1.43 times of cisplatin and 5-FU, respectively. YFCC showed the 
      highest apoptotic effect against KB cells at 10.70 +/- 2.15% at 0.5 mg/mL, which 
      was 0.68 and 1.07 times of cisplatin and 5-FU, respectively. The major 
      phytoconstituents in YFCH were hexadecanoic acid (11.53%) and ethyl oleate 
      (10.58%). DISCUSSION AND CONCLUSION: The crude extracts of Longkong showed 
      anticancer activities and may provide new lead compounds for the development of 
      anticancer products.
FAU - Manosroi, Aranya
AU  - Manosroi A
AD  - Natural Products Research and Development Center (NPRDC), Science and Technology 
      Research Institute (STRI), Chiang Mai University, Chiang Mai 50200, Thailand. 
      pmpti005@chiangmai.ac.th
FAU - Jantrawut, Pensak
AU  - Jantrawut P
FAU - Sainakham, Mathukorn
AU  - Sainakham M
FAU - Manosroi, Worapaka
AU  - Manosroi W
FAU - Manosroi, Jiradej
AU  - Manosroi J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120911
PL  - England
TA  - Pharm Biol
JT  - Pharmaceutical biology
JID - 9812552
RN  - 0 (Antineoplastic Agents, Phytogenic)
RN  - 0 (Flavonoids)
RN  - 0 (Phenols)
RN  - 0 (Plant Extracts)
RN  - Q20Q21Q62J (Cisplatin)
RN  - U3P01618RT (Fluorouracil)
SB  - IM
MH  - Animals
MH  - Antineoplastic Agents, Phytogenic/administration & dosage/isolation & 
      purification/*pharmacology
MH  - Apoptosis/drug effects
MH  - Cell Line, Tumor
MH  - Cell Proliferation/drug effects
MH  - Cisplatin/pharmacology
MH  - Flavonoids/isolation & purification/pharmacology
MH  - Fluorouracil/pharmacology
MH  - Fruit
MH  - Gas Chromatography-Mass Spectrometry
MH  - Humans
MH  - Inhibitory Concentration 50
MH  - Meliaceae/*chemistry
MH  - Mice
MH  - Neoplasms/*drug therapy/pathology
MH  - Phenols/isolation & purification/pharmacology
MH  - Plant Extracts/administration & dosage/*pharmacology
MH  - Thailand
EDAT- 2012/08/02 06:00
MHDA- 2013/03/02 06:00
CRDT- 2012/08/02 06:00
PHST- 2012/08/02 06:00 [entrez]
PHST- 2012/08/02 06:00 [pubmed]
PHST- 2013/03/02 06:00 [medline]
AID - 10.3109/13880209.2012.682116 [doi]
PST - ppublish
SO  - Pharm Biol. 2012 Nov;50(11):1397-407. doi: 10.3109/13880209.2012.682116. Epub 
      2012 Sep 11.