PMID- 22850405
OWN - NLM
STAT- MEDLINE
DCOM- 20130111
LR  - 20211021
IS  - 1559-7016 (Electronic)
IS  - 0271-678X (Print)
IS  - 0271-678X (Linking)
VI  - 32
IP  - 11
DP  - 2012 Nov
TI  - Transport of biogenic amine neurotransmitters at the mouse blood-retina and 
      blood-brain barriers by uptake1 and uptake2.
PG  - 1989-2001
LID - 10.1038/jcbfm.2012.109 [doi]
AB  - Uptake1 and uptake2 transporters are involved in the extracellular clearance of 
      biogenic amine neurotransmitters at synaptic clefts. We looked for them at the 
      blood-brain barrier (BBB) and blood-retina barriers (BRB), where they could be 
      involved in regulating the neurotransmitter concentration and modulate/terminate 
      receptor-mediated effects within the neurovascular unit (NVU). Uptake2 
      (Oct1-3/Slc22a1-3, Pmat/Slc29a4) and Mate1/Slc47a1 transporters are also involved 
      in the transport of xenobiotics. We used in situ carotid perfusion of prototypic 
      substrates like [(3)H]-1-methyl-4-phenylpyridinium ([(3)H]-MPP(+)), 
      [(3)H]-histamine, [(3)H]-serotonin, and [(3)H]-dopamine, changes in ionic 
      composition and genetic deletion of Oct1-3 carriers to detect uptake1 and uptake2 
      at the BBB and BRB. We showed that uptake1 and uptake2 are involved in the 
      transport of [(3)H]-dopamine and [(3)H]-MPP(+) at the blood luminal BRB, but not 
      at the BBB. These functional studies, together with quantitative RT-PCR and 
      confocal imaging, suggest that the mouse BBB lacks uptake1 (Net/Slc6a2, 
      Dat/Slc6a3, Sert/Slc6a4), uptake2, and Mate1 on both the luminal and abluminal 
      sides. However, we found evidence for functional Net and Oct1 transporters at the 
      luminal BRB. These heterogeneous transport properties of the brain and retina 
      NVUs suggest that the BBB helps protect the brain against biogenic amine 
      neurotransmitters in the plasma while the BRB has more of a metabolic/endocrine 
      role.
FAU - Andre, Pascal
AU  - Andre P
AD  - INSERM U705, CNRS UMR 8206, Universite Paris Descartes, Sorbonne Paris Cite, 
      Universite Paris Diderot, Faculte de Pharmacie, Paris, France.
FAU - Saubamea, Bruno
AU  - Saubamea B
FAU - Cochois-Guegan, Veronique
AU  - Cochois-Guegan V
FAU - Marie-Claire, Cynthia
AU  - Marie-Claire C
FAU - Cattelotte, Julie
AU  - Cattelotte J
FAU - Smirnova, Maria
AU  - Smirnova M
FAU - Schinkel, Alfred H
AU  - Schinkel AH
FAU - Scherrmann, Jean-Michel
AU  - Scherrmann JM
FAU - Cisternino, Salvatore
AU  - Cisternino S
LA  - eng
PT  - Journal Article
DEP - 20120801
PL  - United States
TA  - J Cereb Blood Flow Metab
JT  - Journal of cerebral blood flow and metabolism : official journal of the 
      International Society of Cerebral Blood Flow and Metabolism
JID - 8112566
RN  - 0 (Biogenic Amines)
RN  - 0 (Carrier Proteins)
RN  - 0 (Membrane Transport Proteins)
RN  - 0 (Neurotransmitter Agents)
RN  - 333DO1RDJY (Serotonin)
RN  - 63231-63-0 (RNA)
RN  - VTD58H1Z2X (Dopamine)
SB  - IM
MH  - Algorithms
MH  - Animals
MH  - Biogenic Amines/*metabolism
MH  - Blood-Brain Barrier/*metabolism
MH  - Capillaries/metabolism
MH  - Carrier Proteins/metabolism
MH  - Cerebral Cortex/blood supply/metabolism
MH  - Dopamine/metabolism
MH  - Immunochemistry
MH  - Immunohistochemistry
MH  - Kidney/metabolism
MH  - Male
MH  - Membrane Transport Proteins/metabolism
MH  - Mice
MH  - Microscopy, Confocal
MH  - Neurons/metabolism
MH  - Neurotransmitter Agents/*metabolism
MH  - Polymerase Chain Reaction
MH  - RNA/biosynthesis
MH  - Retina/*metabolism
MH  - Serotonin/metabolism
MH  - Tissue Distribution
PMC - PMC3493996
EDAT- 2012/08/02 06:00
MHDA- 2013/01/12 06:00
PMCR- 2013/11/01
CRDT- 2012/08/02 06:00
PHST- 2012/08/02 06:00 [entrez]
PHST- 2012/08/02 06:00 [pubmed]
PHST- 2013/01/12 06:00 [medline]
PHST- 2013/11/01 00:00 [pmc-release]
AID - jcbfm2012109 [pii]
AID - 10.1038/jcbfm.2012.109 [doi]
PST - ppublish
SO  - J Cereb Blood Flow Metab. 2012 Nov;32(11):1989-2001. doi: 10.1038/jcbfm.2012.109. 
      Epub 2012 Aug 1.