PMID- 22851248
OWN - NLM
STAT- MEDLINE
DCOM- 20130318
LR  - 20121005
IS  - 1525-3163 (Electronic)
IS  - 0021-8812 (Linking)
VI  - 90
IP  - 10
DP  - 2012 Oct
TI  - Supplemental palmitoleic (C16:1 cis-9) acid reduces lipogenesis and desaturation 
      in bovine adipocyte cultures.
PG  - 3433-41
LID - 10.2527/jas.2011-4972 [doi]
AB  - Our objective was to determine if palmitoleic (C16:1 cis-9) acid supplementation 
      to primary bovine adipocytes regulates lipogenic gene expression and rates of 
      lipogenesis. Stromal vascular cells were isolated from subcutaneous and 
      intermuscular fat, propagated, and frozen for use in this study. Cells were 
      passaged 4 times, allowed to reach confluence, held for 2 d, and then 
      differentiated with a standard hormone cocktail (d 0). At d 2, secondary 
      differentiation media containing 1 of 4 concentrations of palmitoleic acid (0, 
      50, 150, or 300 muM) were added for 10 d. Cells were harvested on d 6 and 12 to 
      assess fatty acid concentrations and gene expression. In addition, (13)C2 and 
      (13)C18:0 stable isotopes were added on d 6 to measure lipogenesis and desaturase 
      activity, respectively. Concentrations of C16:1 and total fatty acids increased 
      (P < 0.05) linearly in response to palmitoleic acid supplement. Concentrations of 
      C18:1 cis-11 and C20:1 cis-13 also increased (P < 0.01) in response to 
      supplementation, suggesting elongation of palmitoleic acid in vitro. 
      Concentrations of C16:1, C18:1 cis-11, and total fatty acids were also greater (P 
      < 0.05) at d 12 compared with d 6. In contrast, C16:0, C18:0, and C18:1 cis-9 
      concentrations decreased (P < 0.05) in response to palmitoleic acid 
      supplementation and were not affected (P > 0.05) by harvest day. The ratio of 
      C18:1 cis-9/C18:0 and fractional synthetic rate (FSR) of desaturation decreased 
      (P < 0.05) in response to increasing palmitoleic acid supplementation. In 
      addition, FSR of lipogenesis was reduced (P < 0.05) in palmitoleic acid-treated 
      cells. Messenger RNA abundance as determined by real-time quantitative PCR for 
      stearoyl-CoA desaturase 1 (SCD1), fatty acid synthase (FASN), and elongase 
      protein 6 (ELOVL6) genes were reduced (P < 0.05) by palmitoleic acid 
      supplementation. Expression of a beta-oxidation gene, carnitine palmitoyltransferase 
      1A (CPT1A), was upregulated (P < 0.05) with palmitoleic acid supplementation in a 
      dose-responsive manner. Supplementation of palmitoleic acid to bovine adipocytes 
      results in increased incorporation of this fatty acid and its elongation products 
      into the adipocyte, which downregulates SCD1, FASN, and ELOVL6 to decrease 
      lipogenesis and upregulates CPT1A, potentially increasing beta-oxidation. These 
      results suggest that palmitoleic acid, an end product of desaturation, can act as 
      a regulator of lipogenesis, desaturation, and beta-oxidation in bovine adipocytes.
FAU - Burns, T A
AU  - Burns TA
AD  - Department of Animal and Veterinary Sciences, Clemson University, Clemson, SC 
      29634-0311, USA.
FAU - Duckett, S K
AU  - Duckett SK
FAU - Pratt, S L
AU  - Pratt SL
FAU - Jenkins, T C
AU  - Jenkins TC
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20120731
PL  - United States
TA  - J Anim Sci
JT  - Journal of animal science
JID - 8003002
RN  - 0 (Fatty Acids, Monounsaturated)
RN  - 0 (RNA, Messenger)
RN  - 209B6YPZ4I (palmitoleic acid)
SB  - IM
MH  - Adipocytes/*metabolism
MH  - Animals
MH  - Cattle/*metabolism
MH  - Cells, Cultured
MH  - Chromatography, Gas
MH  - Fatty Acids, Monounsaturated/*metabolism
MH  - *Gene Expression Regulation
MH  - *Lipogenesis
MH  - *Lipolysis
MH  - RNA, Messenger/analysis
MH  - Real-Time Polymerase Chain Reaction
EDAT- 2012/08/02 06:00
MHDA- 2013/03/19 06:00
CRDT- 2012/08/02 06:00
PHST- 2012/08/02 06:00 [entrez]
PHST- 2012/08/02 06:00 [pubmed]
PHST- 2013/03/19 06:00 [medline]
AID - jas.2011-4972 [pii]
AID - 10.2527/jas.2011-4972 [doi]
PST - ppublish
SO  - J Anim Sci. 2012 Oct;90(10):3433-41. doi: 10.2527/jas.2011-4972. Epub 2012 Jul 
      31.