PMID- 22851370
OWN - NLM
STAT- MEDLINE
DCOM- 20121203
LR  - 20191210
IS  - 1942-7611 (Electronic)
IS  - 1942-7603 (Linking)
VI  - 4 Suppl 1
DP  - 2012 Aug
TI  - Validation of a new screening, determinative, and confirmatory multi-residue 
      method for nitroimidazoles and their hydroxy metabolites in turkey muscle tissue 
      by liquid chromatography-tandem mass spectrometry.
PG  - 130-8
LID - 10.1002/dta.1356 [doi]
AB  - A new and sensitive multi-residue method (MRM) with detection by LC-MS/MS was 
      developed and validated for the screening, determination, and confirmation of 
      residues of 7 nitroimidazoles and 3 of their metabolites in turkey muscle tissues 
      at concentrations >/= 0.05 ng/g. The compounds were extracted into a solvent with 
      an alkali salt. Sample clean-up and concentration was then done by solid-phase 
      extraction (SPE) and the compounds were quantified by liquid 
      chromatography-tandem mass spectrometry (LC-MS/MS). The characteristic parameters 
      including repeatability, selectivity, ruggedness, stability, level of 
      quantification, and level of confirmation for the new method were determined. 
      Method validation was achieved by independent verification of the parameters 
      measured during method characterization. The seven nitroimidazoles included are 
      metronidazole (MTZ), ronidazole (RNZ), dimetridazole (DMZ), tinidazole (TNZ), 
      ornidazole (ONZ), ipronidazole (IPR), and carnidazole (CNZ). It was discovered 
      during the single laboratory validation of the method that five of the seven 
      nitroimidazoles (i.e. metronidazole, dimetridazole, tinidazole, ornidazole and 
      ipronidazole) and the 3 metabolites 
      (1-(2-hydroxyethyl)-2-hydroxymethyl-5-nitroimidazole (MTZ-OH), 
      2-hydroxymethyl-1-methyl-5-nitroimidazole (HMMNI, the common metabolite of 
      ronidazole and dimetridazole), and 
      1-methyl-2-(2'-hydroxyisopropyl)-5-nitroimidazole (IPR-OH) included in this study 
      could be detected, confirmed, and quantified accurately whereas RNZ and CNZ could 
      only be detected and confirmed but not accurately quantified.
CI  - (c) Her Majesty the Queen in Right of Canada as Represented by the Minister of 
      Agriculture and Agri-food Canada 2012.
FAU - Boison, Joe O
AU  - Boison JO
AD  - Canadian Food Inspection Agency, Saskatoon, Saskatchewan, Canada.
FAU - Asea, Philip A
AU  - Asea PA
FAU - Matus, Johanna L
AU  - Matus JL
LA  - eng
PT  - Journal Article
PT  - Validation Study
PL  - England
TA  - Drug Test Anal
JT  - Drug testing and analysis
JID - 101483449
RN  - 0 (Nitroimidazoles)
SB  - IM
MH  - Animals
MH  - Chromatography, Liquid/methods
MH  - Drug Residues/*analysis/metabolism
MH  - Limit of Detection
MH  - Muscles/chemistry
MH  - Nitroimidazoles/*analysis/metabolism
MH  - Poultry Products/*analysis
MH  - Solid Phase Extraction/methods
MH  - Tandem Mass Spectrometry/*methods
MH  - Turkeys
EDAT- 2012/08/08 06:00
MHDA- 2012/12/10 06:00
CRDT- 2012/08/02 06:00
PHST- 2012/08/02 06:00 [entrez]
PHST- 2012/08/08 06:00 [pubmed]
PHST- 2012/12/10 06:00 [medline]
AID - 10.1002/dta.1356 [doi]
PST - ppublish
SO  - Drug Test Anal. 2012 Aug;4 Suppl 1:130-8. doi: 10.1002/dta.1356.