PMID- 22851387
OWN - NLM
STAT- MEDLINE
DCOM- 20130507
LR  - 20230614
IS  - 1538-2443 (Electronic)
IS  - 1355-0284 (Print)
IS  - 1355-0284 (Linking)
VI  - 18
IP  - 6
DP  - 2012 Dec
TI  - Human trigeminal ganglionic explants as a model to study alphaherpesvirus 
      reactivation.
PG  - 456-61
LID - 10.1007/s13365-012-0123-0 [doi]
AB  - Varicella zoster virus (VZV) latency is characterized by limited virus gene 
      expression and the absence of virus DNA replication. Investigations of VZV 
      latency and reactivation have been hindered by the lack of an in vitro model of 
      virus latency. Since VZV is an exclusively human pathogen, we used naturally 
      infected human trigeminal ganglia (TG) obtained at autopsy to study virus 
      latency. Herein, we report optimization of medium to maintain TG integrity as 
      determined by histology and immunohistochemistry. Using the optimized culture 
      medium, we also found that both herpes simplex virus-1 (HSV-1) and VZV DNA 
      replicated in TG explants after 5 days in culture. The increase in HSV-1 DNA was 
      fourfold greater than the increase in VZV DNA. Overall, we present a model for 
      alphaherpesvirus latency in human neurons in which the key molecular events 
      leading to virus reactivation can be studied.
FAU - Azarkh, Yevgeniy
AU  - Azarkh Y
AD  - Department of Neurology, University of Colorado Denver Medical School, Aurora, 
      CO, USA.
FAU - Bos, Nathan
AU  - Bos N
FAU - Gilden, Don
AU  - Gilden D
FAU - Cohrs, Randall J
AU  - Cohrs RJ
LA  - eng
GR  - P01 AG032958/AG/NIA NIH HHS/United States
GR  - AG032958/AG/NIA NIH HHS/United States
GR  - T32 NS007321/NS/NINDS NIH HHS/United States
GR  - NS007321/NS/NINDS NIH HHS/United States
GR  - R01 AG006127/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20120801
PL  - United States
TA  - J Neurovirol
JT  - Journal of neurovirology
JID - 9508123
RN  - 0 (Culture Media)
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Autopsy
MH  - Culture Media
MH  - DNA, Viral/genetics
MH  - Herpesvirus 1, Human/genetics/*physiology
MH  - Herpesvirus 3, Human/genetics/*physiology
MH  - Humans
MH  - Immunohistochemistry
MH  - Models, Biological
MH  - Polymerase Chain Reaction
MH  - Tissue Culture Techniques
MH  - Trigeminal Ganglion/*ultrastructure/*virology
MH  - Virus Latency
MH  - Virus Replication
PMC - PMC3584453
MID - NIHMS444558
COIS- Conflict of interest The authors report no conflicts of interest.
EDAT- 2012/08/02 06:00
MHDA- 2013/05/08 06:00
PMCR- 2013/12/01
CRDT- 2012/08/02 06:00
PHST- 2012/06/26 00:00 [received]
PHST- 2012/07/11 00:00 [accepted]
PHST- 2012/07/09 00:00 [revised]
PHST- 2012/08/02 06:00 [entrez]
PHST- 2012/08/02 06:00 [pubmed]
PHST- 2013/05/08 06:00 [medline]
PHST- 2013/12/01 00:00 [pmc-release]
AID - 10.1007/s13365-012-0123-0 [doi]
PST - ppublish
SO  - J Neurovirol. 2012 Dec;18(6):456-61. doi: 10.1007/s13365-012-0123-0. Epub 2012 
      Aug 1.