PMID- 22852807
OWN - NLM
STAT- MEDLINE
DCOM- 20120926
LR  - 20151119
IS  - 0894-0282 (Print)
IS  - 0894-0282 (Linking)
VI  - 25
IP  - 9
DP  - 2012 Sep
TI  - Cellulases belonging to glycoside hydrolase families 6 and 7 contribute to the 
      virulence of Magnaporthe oryzae.
PG  - 1135-41
LID - 10.1094/MPMI-02-12-0043-R [doi]
AB  - Upon infection, phytopathogenic fungi secrete an array of hydrolytic enzymes that 
      can degrade components of the host epidermis, including waxes, the cuticle, and 
      cell walls. Cellulases, which can hydrolyze crystalline cellulose in the plant 
      cell wall, are among these hydrolytic enzymes. Here, we provide RNAi-based 
      evidence to show that cellulases belonging to glycosyl hydrolase (GH) families 6 
      and 7 contribute to the penetration of the host epidermis and further invasion by 
      the phytopathogenic fungus Magnaporthe oryzae. The GH6 and GH7 cellulases likely 
      include all members of the cellobiohydrolase family and some endoglucanases in M. 
      oryzae. Quantitative reverse-transcriptase polymerase chain reaction analysis 
      indicated that more than half of the cellulases were highly induced during 
      infection. We constructed knock-down (KD) mutants of these cellulases using the 
      building blocks method we reported previously. The transcript levels of the 
      target genes and cellulase activity were considerably reduced in the KD mutants. 
      The KD mutants resulted in fewer lesions, less penetration, and infection of 
      fewer cells compared with the parent strain. Cytological analyses showed that a 
      high rate of papilla formation blocked invasion of the KD mutants into host 
      cells. These results suggest that the GH6 and GH7 cellulases play roles in the 
      virulence of M. oryzae.
FAU - Van Vu, Ba
AU  - Van Vu B
AD  - Kobe University, Kobe, Japan.
FAU - Itoh, Kenji
AU  - Itoh K
FAU - Nguyen, Quoc Bao
AU  - Nguyen QB
FAU - Tosa, Yukio
AU  - Tosa Y
FAU - Nakayashiki, Hitoshi
AU  - Nakayashiki H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Plant Microbe Interact
JT  - Molecular plant-microbe interactions : MPMI
JID - 9107902
RN  - EC 3.2.1.- (Glycoside Hydrolases)
SB  - IM
MH  - Gene Expression Regulation, Enzymologic
MH  - Gene Expression Regulation, Fungal
MH  - Glycoside Hydrolases/*classification/*metabolism
MH  - Magnaporthe/*enzymology/pathogenicity
MH  - Mutation
MH  - Oryza/*microbiology
MH  - Plant Diseases
MH  - Virulence
EDAT- 2012/08/03 06:00
MHDA- 2012/09/27 06:00
CRDT- 2012/08/03 06:00
PHST- 2012/08/03 06:00 [entrez]
PHST- 2012/08/03 06:00 [pubmed]
PHST- 2012/09/27 06:00 [medline]
AID - 10.1094/MPMI-02-12-0043-R [doi]
PST - ppublish
SO  - Mol Plant Microbe Interact. 2012 Sep;25(9):1135-41. doi: 
      10.1094/MPMI-02-12-0043-R.