PMID- 22853554
OWN - NLM
STAT- MEDLINE
DCOM- 20130625
LR  - 20161125
IS  - 1607-8438 (Electronic)
IS  - 0300-8207 (Linking)
VI  - 54
IP  - 1
DP  - 2013
TI  - Effect of ulinastatin on the expression of iNOS, MMP-2, and MMP-3 in degenerated 
      nucleus pulposus cells of rabbits.
PG  - 29-33
LID - 10.3109/03008207.2012.716882 [doi]
AB  - We examined the effects of ulinastatin on the expression of inducible nitric 
      oxide synthase (iNOS), matrix metalloproteinase-2 (MMP-2), and matrix 
      metalloproteinase-3 (MMP-3) in degenerated nucleus pulposus (NP) cells of rabbits 
      induced by interleukin (IL)-1beta in vitro. An in vitro NP cell culture model was 
      set up with enzyme digestion. NP cells from adult white rabbits were divided into 
      six groups: the normal control group, the ulinastatin control group (320 U/mL 
      ulinastatin), the induced group (10 ng/mL IL-1beta), and three inhibition groups 
      (IL-1beta followed by 160, 320, or 640 U/mL ulinastatin). After a 2-day culture, the 
      NP cells were collected for immunohistochemical staining for MMP-2 and MMP-3 and 
      spectrophotometric analysis of the amount of iNOS. Immunohistochemical staining 
      showed that the expression of MMP-2 and MMP-3 proteins in NP cells decreased in 
      the inhibition groups compared with the induced group, which was in inverse 
      proportion to the ulinastatin concentration. Spectrophotometric results showed 
      that, compared with the induced group, the iNOS content in each inhibition group 
      decreased, most significantly in the 320 U/mL group. Ulinastatin effectively 
      inhibited the increased expression of MMP-2, MMP-3, and iNOS in degenerated NP 
      cells induced by IL-1beta in vitro. It suggests that ulinastatin may potentially be 
      useful for clinical therapy of intervertebral disc degeneration.
FAU - Hua, Guo
AU  - Hua G
AD  - Department of Spine Surgery, Red Cross Hospital Affiliated of Xi'an Jiaotong 
      University, Xi'an, PR China.
FAU - Haiping, Zhang
AU  - Haiping Z
FAU - Baorong, He
AU  - Baorong H
FAU - Dingjun, Hao
AU  - Dingjun H
LA  - eng
PT  - Journal Article
DEP - 20120904
PL  - England
TA  - Connect Tissue Res
JT  - Connective tissue research
JID - 0365263
RN  - 0 (Glycoproteins)
RN  - 0 (Matrix Metalloproteinase Inhibitors)
RN  - 0 (Trypsin Inhibitors)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - OR3S9IF86U (urinastatin)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Disease Models, Animal
MH  - Glycoproteins/*pharmacology
MH  - Immunohistochemistry
MH  - Intervertebral Disc Degeneration/*drug therapy/enzymology/pathology
MH  - Matrix Metalloproteinase 2/*metabolism
MH  - Matrix Metalloproteinase 3/*metabolism
MH  - Matrix Metalloproteinase Inhibitors/pharmacology
MH  - Nitric Oxide Synthase Type II/antagonists & inhibitors/*metabolism
MH  - Rabbits
MH  - Spectrophotometry
MH  - Trypsin Inhibitors/*pharmacology
EDAT- 2012/08/03 06:00
MHDA- 2013/06/26 06:00
CRDT- 2012/08/03 06:00
PHST- 2012/08/03 06:00 [entrez]
PHST- 2012/08/03 06:00 [pubmed]
PHST- 2013/06/26 06:00 [medline]
AID - 10.3109/03008207.2012.716882 [doi]
PST - ppublish
SO  - Connect Tissue Res. 2013;54(1):29-33. doi: 10.3109/03008207.2012.716882. Epub 
      2012 Sep 4.