PMID- 22855265
OWN - NLM
STAT- MEDLINE
DCOM- 20140225
LR  - 20220408
IS  - 1436-3771 (Electronic)
IS  - 1432-6981 (Linking)
VI  - 17
IP  - 3
DP  - 2013 Apr
TI  - In vitro investigation of fluorescence of carious dentin observed with a 
      Soprolife(R) camera.
PG  - 757-63
LID - 10.1007/s00784-012-0770-9 [doi]
AB  - OBJECTIVES: Our aim was to determine the origin of the red fluorescence of 
      carious dentine observed with the Soprolife(R) camera. METHODS: We conducted in 
      vitro studies to evaluate the origin of the red fluorescence using acids and 
      matrix metalloproteinase (MMP) to mimic caries and methylglycoxal (MGO) to 
      evaluate the effect of glycation reactions on the red fluorescence. In every step 
      of these models, we detected the changes of dentin photonic response with 
      Soprolife(R) in daylight mode and in treatment mode. A Raman spectroscopy analysis 
      was performed to determine the variations of the dentin organic during the in 
      vitro caries processes. Raman microscopy was performed to identify change in the 
      collagen matrix of dentine. RESULTS: The red fluorescence observed in carious 
      dentine using a Soprolife(R) camera corresponds to the brownish color observed 
      using daylight. Demineralization using nitric acid induces a loss of the green 
      fluorescence of dentine. The red fluorescence of carious dentine is resistant to 
      acid treatment. Immersion of demineralized dentine in MGO induces a change of 
      color from white to orange-red. This indicates that the Maillard reaction 
      contributes to lesion coloration. Immersion of demineralized dentine in an MMP-1 
      solution followed by MGO treatment results in a similar red fluorescence. Raman 
      microspectroscopy analysis reveals accumulation of AGE's product in red-colored 
      dentine. CONCLUSIONS: Our results provide important information on the origin of 
      the fluorescence variation of dentine observed with the Soprolife(R) camera. We 
      demonstrate that the red fluorescence of carious dentine is linked to the 
      accumulation of Advanced Glycation End products (AGE). CLINICAL RELEVANCE: The 
      study provides a new biological basis for the red fluorescence of carious dentine 
      and reinforces the importance of the Soprolife(R) camera in caries diagnostics.
FAU - Panayotov, Ivan
AU  - Panayotov I
AD  - EA4203 Laboratory of Biology and Nano-science, Universite Montpellier 1, 
      Montpellier, France.
FAU - Terrer, Elodie
AU  - Terrer E
FAU - Salehi, Hamideh
AU  - Salehi H
FAU - Tassery, Herve
AU  - Tassery H
FAU - Yachouh, Jacques
AU  - Yachouh J
FAU - Cuisinier, Frederic J G
AU  - Cuisinier FJ
FAU - Levallois, Bernard
AU  - Levallois B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120802
PL  - Germany
TA  - Clin Oral Investig
JT  - Clinical oral investigations
JID - 9707115
RN  - 0 (Glycation End Products, Advanced)
RN  - 9007-34-5 (Collagen)
RN  - EC 3.4.24.7 (Matrix Metalloproteinase 1)
MH  - Collagen/chemistry
MH  - Dental Caries/diagnosis/*pathology
MH  - Dentin/chemistry/*pathology
MH  - Fluorescence
MH  - Glycation End Products, Advanced/analysis
MH  - Humans
MH  - Maillard Reaction
MH  - Matrix Metalloproteinase 1
MH  - *Photography, Dental/instrumentation
MH  - Spectrum Analysis, Raman
EDAT- 2012/08/03 06:00
MHDA- 2014/02/26 06:00
CRDT- 2012/08/03 06:00
PHST- 2011/11/04 00:00 [received]
PHST- 2012/06/10 00:00 [accepted]
PHST- 2012/08/03 06:00 [entrez]
PHST- 2012/08/03 06:00 [pubmed]
PHST- 2014/02/26 06:00 [medline]
AID - 10.1007/s00784-012-0770-9 [doi]
PST - ppublish
SO  - Clin Oral Investig. 2013 Apr;17(3):757-63. doi: 10.1007/s00784-012-0770-9. Epub 
      2012 Aug 2.