PMID- 22856599
OWN - NLM
STAT- MEDLINE
DCOM- 20121101
LR  - 20211021
IS  - 1743-422X (Electronic)
IS  - 1743-422X (Linking)
VI  - 9
DP  - 2012 Aug 2
TI  - Identification of nonessential regions of the nsp2 protein of an attenuated 
      vaccine strain (HuN4-F112) of highly pathogenic porcine reproductive and 
      respiratory syndrome virus for replication in marc-145 cell.
PG  - 141
LID - 10.1186/1743-422X-9-141 [doi]
AB  - BACKGROUND: The regions in the middle of nonstructural protein 2 (nsp2) of 
      porcine reproductive and respiratory syndrome virus (PRRSV) have been shown to be 
      nonessential for PRRSV replication, and these nonessential regions are different 
      in various viral strains. FINDING: In this study, the nonessential regions of the 
      nsp2 of an attenuated vaccine strain (HuN4-F112) of highly pathogenic porcine 
      reproductive and respiratory syndrome virus were identified based on an 
      infectious cDNA clone of HuN4-F112. The results demonstrated that the segments of 
      nsp2 [amino acids (aa) 480 to 667] tolerated deletions. Characterization of the 
      mutants demonstrated that those with small deletions did not affect the viral 
      growth on Marc-145 cells, but deletion of these regions led to earlier PRRSV 
      replication increased (before 36 h after infectious in vitro). CONCLUSION: The 
      functional roles of nsp2 variable middle region for PRRSV HuN4-F112 replication 
      have been identified. Our results also suggested that none-essential region might 
      be an ideal insertion region to express foreign gene in PRRSV genome.
FAU - Xu, Yan-Zhao
AU  - Xu YZ
AD  - Division of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, 518 Ziyue Road, Minhang District, 
      Shanghai 200241, China.
FAU - Zhou, Yan-Jun
AU  - Zhou YJ
FAU - Zhang, Shan-Rui
AU  - Zhang SR
FAU - Tong, Wu
AU  - Tong W
FAU - Li, Ling
AU  - Li L
FAU - Jiang, Yi-Feng
AU  - Jiang YF
FAU - Tong, Guang-Zhi
AU  - Tong GZ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120802
PL  - England
TA  - Virol J
JT  - Virology journal
JID - 101231645
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA, Viral)
RN  - 0 (Vaccines, Attenuated)
RN  - 0 (Viral Nonstructural Proteins)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - DNA Mutational Analysis
MH  - DNA, Complementary/genetics
MH  - DNA, Viral/genetics
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*growth & 
      development
MH  - Vaccines, Attenuated/genetics
MH  - Viral Nonstructural Proteins/*genetics/*metabolism
PMC - PMC3422162
EDAT- 2012/08/04 06:00
MHDA- 2012/11/02 06:00
PMCR- 2012/08/02
CRDT- 2012/08/04 06:00
PHST- 2011/11/07 00:00 [received]
PHST- 2012/07/25 00:00 [accepted]
PHST- 2012/08/04 06:00 [entrez]
PHST- 2012/08/04 06:00 [pubmed]
PHST- 2012/11/02 06:00 [medline]
PHST- 2012/08/02 00:00 [pmc-release]
AID - 1743-422X-9-141 [pii]
AID - 10.1186/1743-422X-9-141 [doi]
PST - epublish
SO  - Virol J. 2012 Aug 2;9:141. doi: 10.1186/1743-422X-9-141.