PMID- 22857743
OWN - NLM
STAT- MEDLINE
DCOM- 20130212
LR  - 20220318
IS  - 1756-0500 (Electronic)
IS  - 1756-0500 (Linking)
VI  - 5
DP  - 2012 Aug 2
TI  - Nature of bacterial colonization influences transcription of mucin genes in mice 
      during the first week of life.
PG  - 402
LID - 10.1186/1756-0500-5-402 [doi]
AB  - BACKGROUND: Postnatal regulation of the small intestinal mucus layer is 
      potentially important in the development of adult gut functionality. We 
      hypothesized that the nature of bacterial colonization affects mucus gene 
      regulation in early life.We thus analyzed the influence of the presence of a 
      conventional microbiota as well as two selected monocolonizing bacterial strains 
      on the transcription of murine genes involved in mucus layer development during 
      the first week of life.Mouse pups (N = 8/group) from differently colonized dams: 
      Germ-free (GF), conventional specific pathogen free (SPF), monocolonized with 
      either Lactobacillus acidophilus NCFM (Lb) or Escherichia coli Nissle (Ec) were 
      analyzed by qPCR on isolated ileal tissue sections from postnatal days 1 and 6 
      (PND1, PND6) after birth with respect to: (i) transcription of specific genes 
      involved in mucus production (Muc1-4, Tff3) and (ii) amounts of 16S rRNA of 
      Lactobacillus and E. coli. Quantification of 16S rRNA genes was performed to 
      obtain a measure for amounts of colonized bacteria. RESULTS: We found a 
      microbiota-independent transcriptional increase of all five mucus genes from PND1 
      to PND6. Furthermore, the relative level of transcription of certain mucus genes 
      on PND1 was increased by the presence of bacteria. This was observed for Tff3 in 
      the SPF, Ec, and Lb groups; for Muc2 in SPF; and for Muc3 and Muc4 in Ec and Lb, 
      respectively.Detection of bacterial 16S rRNA genes levels above the qPCR 
      detection level occurred only on PND6 and only for some of the colonized animals. 
      On PND6, we found significantly lower levels of Muc1, Muc2 and Muc4 gene 
      transcription for Lb animals with detectable Lactobacillus levels as compared to 
      animals with Lactobacillus levels below the detection limit. CONCLUSIONS: In 
      summary, our data show that development of the expression of genes encoding 
      secreted (Muc2/Tff3) and membrane-bound (Muc1/Muc3/Muc4) mucus regulatory 
      proteins, respectively, is distinct and that the onset of this development may be 
      accelerated by specific groups of bacteria present or absent at the mucosal site.
FAU - Bergstrom, Anders
AU  - Bergstrom A
AD  - Gut Ecology Group, Department of Food Microbiology, National Food Institute, 
      Technical University of Denmark, Soborg, Denmark. adbe@food.dtu.dk
FAU - Kristensen, Matilde B
AU  - Kristensen MB
FAU - Bahl, Martin I
AU  - Bahl MI
FAU - Metzdorff, Stine B
AU  - Metzdorff SB
FAU - Fink, Lisbeth N
AU  - Fink LN
FAU - Frokiaer, Hanne
AU  - Frokiaer H
FAU - Licht, Tine R
AU  - Licht TR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120802
PL  - England
TA  - BMC Res Notes
JT  - BMC research notes
JID - 101462768
RN  - 0 (Muc2 protein, mouse)
RN  - 0 (Muc3 protein, mouse)
RN  - 0 (Muc4 protein, mouse)
RN  - 0 (Mucin-1)
RN  - 0 (Mucin-2)
RN  - 0 (Mucin-3)
RN  - 0 (Mucin-4)
RN  - 0 (Mucins)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Tff3 protein, mouse)
RN  - 0 (Trefoil Factor-3)
RN  - 0 (muc1 protein, mouse)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Escherichia coli/*physiology
MH  - *Gene Expression Regulation, Developmental
MH  - Germ-Free Life/genetics
MH  - Intestine, Small/*metabolism/microbiology
MH  - Lactobacillus acidophilus/*physiology
MH  - Metagenome/*physiology
MH  - Mice
MH  - Microbial Consortia/genetics
MH  - Mucin-1/genetics/metabolism
MH  - Mucin-2/genetics/metabolism
MH  - Mucin-3/genetics/metabolism
MH  - Mucin-4/genetics/metabolism
MH  - Mucins/genetics/metabolism
MH  - Mucus/*metabolism/microbiology
MH  - RNA, Ribosomal, 16S/genetics
MH  - Real-Time Polymerase Chain Reaction
MH  - *Transcription, Genetic
MH  - Trefoil Factor-3
PMC - PMC3465226
EDAT- 2012/08/04 06:00
MHDA- 2013/02/13 06:00
PMCR- 2012/08/02
CRDT- 2012/08/04 06:00
PHST- 2011/11/16 00:00 [received]
PHST- 2012/07/12 00:00 [accepted]
PHST- 2012/08/04 06:00 [entrez]
PHST- 2012/08/04 06:00 [pubmed]
PHST- 2013/02/13 06:00 [medline]
PHST- 2012/08/02 00:00 [pmc-release]
AID - 1756-0500-5-402 [pii]
AID - 10.1186/1756-0500-5-402 [doi]
PST - epublish
SO  - BMC Res Notes. 2012 Aug 2;5:402. doi: 10.1186/1756-0500-5-402.