PMID- 22858412
OWN - NLM
STAT- MEDLINE
DCOM- 20121211
LR  - 20120914
IS  - 1879-0089 (Electronic)
IS  - 0145-305X (Linking)
VI  - 38
IP  - 2
DP  - 2012 Oct
TI  - The bioactivity of teleost IL-6: IL-6 protein in orange-spotted grouper 
      (Epinephelus coioides) induces Th2 cell differentiation pathway and antibody 
      production.
PG  - 285-94
LID - S0145-305X(12)00161-9 [pii]
LID - 10.1016/j.dci.2012.06.013 [doi]
AB  - Interleukin 6 (IL-6) is a protein secreted by T cells and macrophages and plays 
      an important role in immune response. IL-6 regulates the proliferation and 
      differentiation of T cells, and elicits immunoglobulin production in B cells. In 
      this study, the cDNA il-6 (gil-6) sequence of the orange spotted grouper 
      (Epinephelus coioides) was obtained. The deduced IL-6 (gIL-6) protein comprised 
      223 amino acids, the sequence shared approximately 30% similarity with mammalian 
      IL-6, and between 47% and 69% similarity with other available teleost IL-6. The 
      protein comprises the signal peptide, the IL-6 family signature, and conserved 
      amino acid residues found in IL-6 sequences of other teleost. In order to 
      understand the bioactivity and influence of gIL-6 on humoral immune response, 
      recombinant gIL-6 (rgIL-6) synthesized by prokaryotes was injected into orange 
      spotted groupers, and the immune-related gene expression at various times in 
      various organs was observed. Our results revealed that the Th1 specific 
      transcription factor t-bet was down-regulated and Th2 specific transcription 
      factors gata3, and c-maf were up-regulated in immune organs, following IL-6 
      stimulation. Additionally, higher levels of igm mRNA and translated protein were 
      detected in rgIL-6 stimulated fish. These results indicate that IL-6 in groupers 
      regulates the differentiation of nasmall yi, Ukrainianve T helper cells into Th2 cells and elicits 
      the production of antibodies.
CI  - Copyright (c) 2012 Elsevier Ltd. All rights reserved.
FAU - Chen, Hsin-Hung
AU  - Chen HH
AD  - Biotechnology and Institute of Biotechnology, National Cheng Kung University, 
      Tainan 701, Taiwan, ROC.
FAU - Lin, Han-Tso
AU  - Lin HT
FAU - Foung, Yi-Fan
AU  - Foung YF
FAU - Han-You Lin, John
AU  - Han-You Lin J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120731
PL  - United States
TA  - Dev Comp Immunol
JT  - Developmental and comparative immunology
JID - 7708205
RN  - 0 (Fish Proteins)
RN  - 0 (Interleukin-6)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibody Formation
MH  - Base Sequence
MH  - Bass/*immunology
MH  - *Cell Differentiation
MH  - Cloning, Molecular
MH  - Fish Proteins/*immunology
MH  - Interleukin-6/*immunology
MH  - Molecular Sequence Data
MH  - Sequence Alignment
MH  - Th2 Cells/*cytology/immunology
EDAT- 2012/08/04 06:00
MHDA- 2012/12/12 06:00
CRDT- 2012/08/04 06:00
PHST- 2012/04/17 00:00 [received]
PHST- 2012/06/20 00:00 [revised]
PHST- 2012/06/20 00:00 [accepted]
PHST- 2012/08/04 06:00 [entrez]
PHST- 2012/08/04 06:00 [pubmed]
PHST- 2012/12/12 06:00 [medline]
AID - S0145-305X(12)00161-9 [pii]
AID - 10.1016/j.dci.2012.06.013 [doi]
PST - ppublish
SO  - Dev Comp Immunol. 2012 Oct;38(2):285-94. doi: 10.1016/j.dci.2012.06.013. Epub 
      2012 Jul 31.