PMID- 22858807
OWN - NLM
STAT- MEDLINE
DCOM- 20130220
LR  - 20131121
IS  - 1364-5528 (Electronic)
IS  - 0003-2654 (Linking)
VI  - 137
IP  - 18
DP  - 2012 Sep 21
TI  - A novel chiral ligand exchange capillary electrophoresis system with amino acid 
      ionic liquid as ligand and its application in screening D-amino-acid oxidase 
      inhibitors.
PG  - 4235-40
LID - 10.1039/c2an35753d [doi]
AB  - A novel amino acid ionic liquid (AAIL) with L-ornithine (L-Orn) as anion was 
      successfully synthesized, and subsequently applied as an available chiral ligand 
      coordinated with Zn(II) in a chiral ligand exchange capillary electrophoresis 
      (CLE-CE) system for the enantioseparation of dansyl amino acids (Dns-D,L-AAs). 
      The influence of key parameters, such as buffer pH, concentration ratio of Zn(II) 
      to ligand and complex concentration, was investigated in detail. Eleven pairs of 
      Dns-D,L-AAs enantiomers were baseline separated and three pairs were partly 
      separated under the optimum conditions. For exploring its potential application, 
      the quantitative features of this proposed method were studied. Good linearity 
      (r(2) = 0.999) and favorable repeatability (RSD </= 3.4%) were obtained by using 
      Dns-D,L-Met as the test analyte. Finally, this method was employed to investigate 
      the inhibition efficiency of d-amino acid oxidase (DAAO) inhibitors, which may 
      pave a new way for the high-throughput screening of enzyme inhibitors and 
      relevant drug discovery.
FAU - Mu, Xiaoyu
AU  - Mu X
AD  - Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, 
      Chinese Academy of Sciences, China.
FAU - Qi, Li
AU  - Qi L
FAU - Shen, Ying
AU  - Shen Y
FAU - Zhang, Haizhi
AU  - Zhang H
FAU - Qiao, Juan
AU  - Qiao J
FAU - Ma, Huimin
AU  - Ma H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120803
PL  - England
TA  - Analyst
JT  - The Analyst
JID - 0372652
RN  - 0 (Amino Acids)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Ionic Liquids)
RN  - 0 (Ligands)
RN  - E524N2IXA3 (Ornithine)
RN  - EC 1.4.3.3 (D-Amino-Acid Oxidase)
RN  - J41CSQ7QDS (Zinc)
SB  - IM
MH  - Amino Acids/analysis/*chemistry/isolation & purification
MH  - D-Amino-Acid Oxidase/*antagonists & inhibitors/metabolism
MH  - Drug Discovery
MH  - Drug Evaluation, Preclinical
MH  - Electrophoresis, Capillary/*methods
MH  - Enzyme Inhibitors/*pharmacology
MH  - Ionic Liquids
MH  - Ligands
MH  - Ornithine
MH  - Stereoisomerism
MH  - Zinc/chemistry
EDAT- 2012/08/04 06:00
MHDA- 2013/02/21 06:00
CRDT- 2012/08/04 06:00
PHST- 2012/08/04 06:00 [entrez]
PHST- 2012/08/04 06:00 [pubmed]
PHST- 2013/02/21 06:00 [medline]
AID - 10.1039/c2an35753d [doi]
PST - ppublish
SO  - Analyst. 2012 Sep 21;137(18):4235-40. doi: 10.1039/c2an35753d. Epub 2012 Aug 3.