PMID- 22861646
OWN - NLM
STAT- MEDLINE
DCOM- 20130131
LR  - 20120921
IS  - 1399-0039 (Electronic)
IS  - 0001-2815 (Linking)
VI  - 80
IP  - 4
DP  - 2012 Oct
TI  - Super high resolution for single molecule-sequence-based typing of classical HLA 
      loci at the 8-digit level using next generation sequencers.
PG  - 305-16
LID - 10.1111/j.1399-0039.2012.01941.x [doi]
AB  - Current human leukocyte antigen (HLA) DNA typing methods such as the 
      sequence-based typing (SBT) and sequence-specific oligonucleotide (SSO) methods 
      generally yield ambiguous typing results because of oligonucleotide probe design 
      limitations or phase ambiguity for HLA allele assignment. Here we describe the 
      development and application of the super high-resolution single-molecule 
      sequence-based typing (SS-SBT) of HLA loci at the 8-digit level using next 
      generation sequencing (NGS). NGS which can determine an HLA allele sequence 
      derived from a single DNA molecule is expected to solve the phase ambiguity 
      problem. Eight classical HLA loci-specific polymerase chain reaction (PCR) 
      primers were designed to amplify the entire gene sequences from the 
      enhancer-promoter region to the 3' untranslated region. Phase ambiguities of 
      HLA-A, -B, -C, -DRB1 and -DQB1 were completely resolved and unequivocally 
      assigned without ambiguity to single HLA alleles. Therefore, the SS-SBT method 
      described here is a superior and effective HLA DNA typing method to efficiently 
      detect new HLA alleles and null alleles without ambiguity.
CI  - (c) 2012 John Wiley & Sons A/S.
FAU - Shiina, T
AU  - Shiina T
AD  - Division of Basic Medical Science and Molecular Medicine, Department of Molecular 
      Life Science, Tokai University School of Medicine, Isehara, Kanagawa, Japan.
FAU - Suzuki, S
AU  - Suzuki S
FAU - Ozaki, Y
AU  - Ozaki Y
FAU - Taira, H
AU  - Taira H
FAU - Kikkawa, E
AU  - Kikkawa E
FAU - Shigenari, A
AU  - Shigenari A
FAU - Oka, A
AU  - Oka A
FAU - Umemura, T
AU  - Umemura T
FAU - Joshita, S
AU  - Joshita S
FAU - Takahashi, O
AU  - Takahashi O
FAU - Hayashi, Y
AU  - Hayashi Y
FAU - Paumen, M
AU  - Paumen M
FAU - Katsuyama, Y
AU  - Katsuyama Y
FAU - Mitsunaga, S
AU  - Mitsunaga S
FAU - Ota, M
AU  - Ota M
FAU - Kulski, J K
AU  - Kulski JK
FAU - Inoko, H
AU  - Inoko H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120804
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (3' Untranslated Regions)
RN  - 0 (DNA Primers)
RN  - 0 (HLA Antigens)
SB  - IM
MH  - 3' Untranslated Regions
MH  - Alleles
MH  - DNA Primers
MH  - *Genetic Loci
MH  - HLA Antigens/*analysis/genetics
MH  - High-Throughput Nucleotide Sequencing/instrumentation/*methods
MH  - Humans
MH  - Multilocus Sequence Typing/instrumentation/*methods
MH  - Polymerase Chain Reaction
MH  - Promoter Regions, Genetic
MH  - Sequence Analysis, DNA
EDAT- 2012/08/07 06:00
MHDA- 2013/02/01 06:00
CRDT- 2012/08/07 06:00
PHST- 2012/05/22 00:00 [received]
PHST- 2012/07/03 00:00 [accepted]
PHST- 2012/08/07 06:00 [entrez]
PHST- 2012/08/07 06:00 [pubmed]
PHST- 2013/02/01 06:00 [medline]
AID - 10.1111/j.1399-0039.2012.01941.x [doi]
PST - ppublish
SO  - Tissue Antigens. 2012 Oct;80(4):305-16. doi: 10.1111/j.1399-0039.2012.01941.x. 
      Epub 2012 Aug 4.