PMID- 22861687
OWN - NLM
STAT- MEDLINE
DCOM- 20130131
LR  - 20120921
IS  - 1399-0039 (Electronic)
IS  - 0001-2815 (Linking)
VI  - 80
IP  - 4
DP  - 2012 Oct
TI  - Evaluation of sequence-specific priming and real-time polymerase chain reaction 
      assays for detecting HLA-B*51 alleles confirmed by sequence-based typing.
PG  - 376-9
LID - 10.1111/j.1399-0039.2012.01942.x [doi]
AB  - The human leukocyte antigen (HLA)-B*51 genotype is one of the well-known genetic 
      factors associated with the development of Behcet's disease. We evaluated three 
      sequence-specific priming (SSP) assays and one real-time PCR assay for detecting 
      HLA-B*51 alleles using 93 whole blood samples, which were genotyped by 
      high-resolution sequence-based typing (SBT). All HLA-B*51 alleles determined by 
      SBT were detected by the four evaluated assays, and the results for all HLA-B 
      alleles other than HLA-B*51 were negative on all assays. Thus, all HLA-B51 tests 
      showed 100% sensitivity and 100% specificity for detecting HLA-B*51 alleles. The 
      three SSP assays and the real-time PCR test for HLA-B*51 genotyping are simple, 
      but reliable for detecting HLA-B*51 alleles in clinical laboratories.
CI  - (c) 2012 John Wiley & Sons A/S.
FAU - Park, Y
AU  - Park Y
AD  - Department of Laboratory Medicine, Yonsei University College of Medicine, 
      Seodaemun-gu, Seoul, South Korea.
FAU - Kim, Y S
AU  - Kim YS
FAU - Kim, S I
AU  - Kim SI
FAU - Kim, H
AU  - Kim H
FAU - Kim, H S
AU  - Kim HS
LA  - eng
PT  - Journal Article
DEP - 20120802
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (HLA-B51 Antigen)
SB  - IM
MH  - *Alleles
MH  - Behcet Syndrome/*diagnosis/*genetics/immunology
MH  - Genotype
MH  - HLA-B51 Antigen/*genetics/immunology
MH  - Histocompatibility Testing/*methods
MH  - Humans
MH  - Real-Time Polymerase Chain Reaction
MH  - Sensitivity and Specificity
MH  - Sequence Analysis, DNA
EDAT- 2012/08/07 06:00
MHDA- 2013/02/01 06:00
CRDT- 2012/08/07 06:00
PHST- 2012/03/22 00:00 [received]
PHST- 2012/06/05 00:00 [revised]
PHST- 2012/07/09 00:00 [accepted]
PHST- 2012/08/07 06:00 [entrez]
PHST- 2012/08/07 06:00 [pubmed]
PHST- 2013/02/01 06:00 [medline]
AID - 10.1111/j.1399-0039.2012.01942.x [doi]
PST - ppublish
SO  - Tissue Antigens. 2012 Oct;80(4):376-9. doi: 10.1111/j.1399-0039.2012.01942.x. 
      Epub 2012 Aug 2.