PMID- 22863941
OWN - NLM
STAT- MEDLINE
DCOM- 20121221
LR  - 20190720
IS  - 1347-5215 (Electronic)
IS  - 0918-6158 (Linking)
VI  - 35
IP  - 8
DP  - 2012
TI  - Pinusolide isolated from Biota orientalis inhibits 5-lipoxygenase dependent 
      leukotriene C4 generation by blocking c-Jun N-terminal kinase pathway in mast 
      cells.
PG  - 1374-8
AB  - Pinusolide, an herbal medicine isolated from Biota orientalis L. (B. orientalis), 
      inhibited 5-lipoxygenase (5-LO)-dependent leukotriene C4 (LTC4) generation in 
      immunoglobulin E (IgE)/Ag-induced bone marrow-derived mast cells (BMMCs) in a 
      concentration-dependent manner. To clarify the action mechanism of pinusolide on 
      the inhibition of LTC4 generation, we examined the effect of pinusolide on 
      phosphorylation of cytosolic phospholipase A2 (cPLA2), as well as translocation 
      phospho-cPLA2 and 5-LO to nucleus. Inhibition of LTC4 generation by pinusolide 
      was accompanied by a decrease in cPLA2 phosphorylation which occurred via a 
      decrease in intracellular Ca2+ influx and blocking the c-Jun N-terminal kinase 
      (JNK) pathways. However, pinusolide had no effect on extracellular 
      signal-regulated kinase (ERK) and p38 mitogen-activated protein (MAP) kinas 
      phosphorylation. Taken together, the present results suggest that potent 
      inhibition of 5-LO dependent LTC4 generation by pinusolide requires both 
      suppression of calcium influx and JNK phosphorylation.
FAU - Jin, Ye
AU  - Jin Y
AD  - College of Pharmacy, Yeungnam University, Gyeongsan 712-749, Republic of Korea.
FAU - Yang, Hyun Ok
AU  - Yang HO
FAU - Son, Jong Keun
AU  - Son JK
FAU - Chang, Hyeun Wook
AU  - Chang HW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Biol Pharm Bull
JT  - Biological & pharmaceutical bulletin
JID - 9311984
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Diterpenes)
RN  - 0 (Plant Extracts)
RN  - 2CU6TT9V48 (Leukotriene C4)
RN  - 31685-80-0 (pinusolide)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - EC 1.13.11.34 (Arachidonate 5-Lipoxygenase)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 3.1.1.4 (Phospholipases A2)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/isolation & purification/pharmacology
MH  - Arachidonate 5-Lipoxygenase/*metabolism
MH  - Biological Transport
MH  - Bone Marrow
MH  - Calcium/*metabolism
MH  - Cell Nucleus
MH  - Cytosol
MH  - Diterpenes/isolation & purification/*pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Extracellular Signal-Regulated MAP Kinases/metabolism
MH  - Immunoglobulin E/metabolism
MH  - JNK Mitogen-Activated Protein Kinases/*metabolism
MH  - Leukotriene C4/*biosynthesis
MH  - Male
MH  - Mast Cells/*drug effects/metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Phospholipases A2/metabolism
MH  - Phosphorylation
MH  - Plant Extracts/chemistry/pharmacology
MH  - Signal Transduction
MH  - Thuja/*chemistry
EDAT- 2012/08/07 06:00
MHDA- 2012/12/22 06:00
CRDT- 2012/08/07 06:00
PHST- 2012/08/07 06:00 [entrez]
PHST- 2012/08/07 06:00 [pubmed]
PHST- 2012/12/22 06:00 [medline]
AID - DN/JST.JSTAGE/bpb/b12-00271 [pii]
AID - 10.1248/bpb.b12-00271 [doi]
PST - ppublish
SO  - Biol Pharm Bull. 2012;35(8):1374-8. doi: 10.1248/bpb.b12-00271.