PMID- 22865009
OWN - NLM
STAT- MEDLINE
DCOM- 20130312
LR  - 20191210
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Linking)
VI  - 404
IP  - 6-7
DP  - 2012 Oct
TI  - Distribution measurements of 3,4-methylenedioxymethamphetamine and its 
      metabolites in organs by matrix-assisted laser desorption/ionization imaging mass 
      spectrometry using an automatic matrix spraying system with an air brush and a 
      turntable.
PG  - 1823-30
LID - 10.1007/s00216-012-6279-x [doi]
AB  - Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI/IMS) 
      is a useful tool for measuring drug distributions. To obtain reproducible 
      analytical results with MALDI/IMS, it is essential to apply a homogeneous matrix 
      coating onto sample surfaces. A simple and inexpensive automatic matrix spraying 
      system (AMSS) with good reproducibility was developed in this study. In addition, 
      drug distributions in organs were measured by MALDI/IMS using the AMSS for 
      forensic toxicology applications. The AMSS was constructed from simple 
      components, including an air brush, a turntable, and a microscope. Organ slices 
      placed onto conductive sheets were attached to the turntable. The trigger of the 
      air brush was held with a clamp to ensure that it sprayed continuously onto a 
      defined area of the table. Periodic spraying of the matrix solution and 
      evaporation of solvent were performed by rotating the turntable. The droplets and 
      crystals on the sample surfaces were observed under a microscope attached to the 
      turntable. The droplet size, rotation rate of the turntable, and the formulation 
      of the matrix solution were optimized. The homogeneity of the matrix coating was 
      evaluated using the coefficients of variation (CV) obtained by quantifying the 
      color density of the sheet surface. The AMSS enabled more homogeneous matrix 
      coating (intersheet CV = 5.4 %) than manual spraying (intersheet CV = 16.7 %) 
      when 10 mL of 0.5 % aqueous trifluoroacetic acid/acetonitrile (1:3, v/v) 
      containing 10 mg/mL alpha-cyano-4-hydroxycinnamic acid were sprayed as droplets less 
      than 50 mum in diameter onto a turntable rotating at 30 rpm. The distributions of 
      3,4-methylenedioxymethamphetamine (MDMA) and its main metabolites in the brain, 
      liver, and kidney of a mouse that died from an MDMA overdose (58 mg/kg i.p.) were 
      visualized by MALDI/IMS using the AMSS. The ion intensities of MDMA obtained from 
      the same regions on three sequential kidney slices showed acceptable variations 
      (CV = 2.9-8.8 % for five different regions), implying repeatable measurements 
      with MALDI/IMS using the AMSS. It was revealed that MDMA was particularly 
      concentrated around the brain stem and the major calix of the kidney. The AMSS 
      would be suitable for preparing samples for measuring the distributions of drugs 
      in organs at toxic dose levels in forensic toxicological applications.
FAU - Kuwayama, Kenji
AU  - Kuwayama K
AD  - National Research Institute of Police Science, Kashiwa, Chiba, Japan. 
      kuwayama@nrips.go.jp
FAU - Tsujikawa, Kenji
AU  - Tsujikawa K
FAU - Miyaguchi, Hajime
AU  - Miyaguchi H
FAU - Kanamori, Tatsuyuki
AU  - Kanamori T
FAU - Iwata, Yuko T
AU  - Iwata YT
FAU - Inoue, Hiroyuki
AU  - Inoue H
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120804
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Psychotropic Drugs)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
SB  - IM
MH  - Animals
MH  - Brain/metabolism
MH  - Brain Chemistry
MH  - Kidney/chemistry/metabolism
MH  - Liver/chemistry/metabolism
MH  - Mice
MH  - N-Methyl-3,4-methylenedioxyamphetamine/*analysis/*pharmacokinetics
MH  - Psychotropic Drugs/*analysis/*pharmacokinetics
MH  - Spectrometry, Mass, Matrix-Assisted Laser 
      Desorption-Ionization/instrumentation/*methods
EDAT- 2012/08/07 06:00
MHDA- 2013/03/13 06:00
CRDT- 2012/08/07 06:00
PHST- 2012/01/04 00:00 [received]
PHST- 2012/07/17 00:00 [accepted]
PHST- 2012/06/19 00:00 [revised]
PHST- 2012/08/07 06:00 [entrez]
PHST- 2012/08/07 06:00 [pubmed]
PHST- 2013/03/13 06:00 [medline]
AID - 10.1007/s00216-012-6279-x [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2012 Oct;404(6-7):1823-30. doi: 10.1007/s00216-012-6279-x. 
      Epub 2012 Aug 4.