PMID- 22868136
OWN - NLM
STAT- MEDLINE
DCOM- 20121206
LR  - 20191210
IS  - 1873-7072 (Electronic)
IS  - 0308-8146 (Linking)
VI  - 135
IP  - 2
DP  - 2012 Nov 15
TI  - Analysis of free and esterified sterols in edible oils by online reversed phase 
      liquid chromatography-gas chromatography (RPLC-GC) using the through oven 
      transfer adsorption desorption (TOTAD) interface.
PG  - 610-5
LID - 10.1016/j.foodchem.2012.04.097 [doi]
AB  - An online reversed phase liquid chromatography-gas chromatography (RPLC-GC) 
      method is proposed to quantify free, total and esterified sterols of edible oils. 
      To determine free sterols the diluted oils are injected into the liquid 
      chromatograph, where free sterols are separated from triglycerides and the sterol 
      fraction is automatically transferred to the gas chromatograph to be analysed. To 
      determine total sterols the samples were saponified with potassium hydroxide in 
      ethanolic solution and the unsaponifiable fraction was extracted with diethyl 
      ether. The extract was then analysed by RPLC-GC, avoiding the laborious thin 
      layer chromatography step used in the Official European Union (EU) Method. The 
      relative standard deviations (RSDs) from the absolute peak area varied from 7.6% 
      to 15.8%. Limits of detection (LODs) were less than 8.5 mg/kg. No variability in 
      retention time was observed. The method was applied to the determination of total 
      sterols in edible oil samples and the results were compared with those obtained 
      with the Official EU Method. Good agreement was found between both methods, 
      except in the case of campesterol.
CI  - Copyright (c) 2012 Elsevier Ltd. All rights reserved.
FAU - Toledano, Rosa M
AU  - Toledano RM
AD  - Facultad de Educacion de Albacete, Departamento de Quimica-Fisica, Universidad de 
      Castilla-La Mancha, Campus Universitario s/n., 02071 Albacete, Spain.
FAU - Cortes, Jose M
AU  - Cortes JM
FAU - Rubio-Moraga, Angela
AU  - Rubio-Moraga A
FAU - Villen, Jesus
AU  - Villen J
FAU - Vazquez, Ana
AU  - Vazquez A
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120430
PL  - England
TA  - Food Chem
JT  - Food chemistry
JID - 7702639
RN  - 0 (Plant Oils)
RN  - 0 (Sterols)
SB  - IM
MH  - Chromatography, Gas/instrumentation/*methods
MH  - Chromatography, Liquid/instrumentation/*methods
MH  - Chromatography, Reverse-Phase/instrumentation/methods
MH  - Esterification
MH  - Online Systems/instrumentation
MH  - Plant Oils/*chemistry
MH  - Sterols/*chemistry
EDAT- 2012/08/08 06:00
MHDA- 2012/12/10 06:00
CRDT- 2012/08/08 06:00
PHST- 2011/04/28 00:00 [received]
PHST- 2011/12/19 00:00 [revised]
PHST- 2012/04/15 00:00 [accepted]
PHST- 2012/08/08 06:00 [entrez]
PHST- 2012/08/08 06:00 [pubmed]
PHST- 2012/12/10 06:00 [medline]
AID - S0308-8146(12)00760-1 [pii]
AID - 10.1016/j.foodchem.2012.04.097 [doi]
PST - ppublish
SO  - Food Chem. 2012 Nov 15;135(2):610-5. doi: 10.1016/j.foodchem.2012.04.097. Epub 
      2012 Apr 30.