PMID- 22870709
OWN - NLM
STAT- MEDLINE
DCOM- 20150516
LR  - 20181201
IS  - 1009-4598 (Print)
IS  - 1009-4598 (Linking)
VI  - 28
IP  - 3
DP  - 2012 May
TI  - [Treat flap ischemia-reperfusion injury by local transplanting human umbilical 
      cord mesenchymal stem cells].
PG  - 203-7
AB  - OBJECTIVE: To explore the possibilities of human umbilical cord mesenchymal stem 
      cells (HUC-MSCs) differentiate into vascular endothelial cells, thus to treat 
      flap ischemic reperfusion injury and then promote the flap survival. METHODS: 
      HUC-MSCs in vitro, and flow cytometric identification. Labeling stem cells with 
      EdU, and then transplant stem cells into local of ischemia-reperfusion injury 
      flap of rats (treatment group) , setting PBS control group. Observe the flap 
      survival rate 7 days after, and execute rats. Take flap tissues for pathological 
      sections, and detect vascular endothelial growth factor (VEGF) by 
      immunohistochemical staining, detect the distribution or differentiation of the 
      donor cells in cell receptor by EdU fluorescent staining. RESULTS: 7 days after, 
      the flap survival rate of treatment group was (97.58 +/- 3.41)%, the flap 
      survival rate of control group was (54.37 +/- 8.78)%, the rate of treatment group 
      was higher than control group (P < 0.05). The density of micrangium and VEGF of 
      treatment group was higher than control group obviously (P < 0.05). The 
      continuous distribution of positive cells that labeled with EdU were found in new 
      vascular endothelial of treatment group' s survival flap tissues. CONCLUSIONS: 
      HUC-MSCs could differentiate into vascular endothelial cells, directly involved 
      in generating new blood vesselsand, additionally could increase expression of 
      VEGF to promote the formation of new blood vessels, then to set up the new micro 
      circulation, accordingly to treat flap ischemic reperfusion injury and then 
      promote the flap survival.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Zheng Xing Wai Ke Za Zhi
JT  - Zhonghua zheng xing wai ke za zhi = Zhonghua zhengxing waike zazhi = Chinese 
      journal of plastic surgery
JID - 100957850
RN  - 0 (Vascular Endothelial Growth Factor A)
SB  - IM
MH  - Animals
MH  - *Cell Differentiation
MH  - Graft Survival/physiology
MH  - Humans
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/*cytology
MH  - Rats
MH  - Reperfusion Injury/*therapy
MH  - Stem Cell Transplantation
MH  - Surgical Flaps/*blood supply
MH  - Umbilical Cord/*cytology
MH  - Vascular Endothelial Growth Factor A/metabolism
EDAT- 2012/08/09 06:00
MHDA- 2015/05/20 06:00
CRDT- 2012/08/09 06:00
PHST- 2012/08/09 06:00 [entrez]
PHST- 2012/08/09 06:00 [pubmed]
PHST- 2015/05/20 06:00 [medline]
PST - ppublish
SO  - Zhonghua Zheng Xing Wai Ke Za Zhi. 2012 May;28(3):203-7.