PMID- 22875340
OWN - NLM
STAT- MEDLINE
DCOM- 20121031
LR  - 20181201
IS  - 1535-3699 (Electronic)
IS  - 1535-3699 (Linking)
VI  - 237
IP  - 8
DP  - 2012 Aug
TI  - Resveratrol promotes neurotrophic factor release from astroglia.
PG  - 943-8
LID - 10.1258/ebm.2012.012044 [doi]
AB  - Neurotrophic factors such as glial cell line-derived neurotrophic factor (GDNF) 
      and brain-derived neurotrophic factor (BDNF) are considered to contribute to the 
      development, maintenance and survival of neurons, glia and oligodendrocytes. 
      Astroglia are a major source of various neurotrophic factors. Thus, enhancement 
      of astroglia-mediated neurotrophic factor release might hold promising potential 
      for neurological diseases. Resveratrol, a natural non-flavonoid polyphenol found 
      in grapes and red wine, has been recognized to be beneficial for health. Here, 
      rat primary astroglia-enriched cultures were used to investigate the effects of 
      resveratrol-mediated neurotrophic factor release and the related mechanisms. The 
      cultures were treated with 25-100 mummol/L resveratrol for 12-48 h. Results showed 
      resveratrol increased BDNF and GDNF production in the culture medium. In 
      addition, the production of BDNF in the supernatant of cultures was increased 
      five-fold over control cultures 24 h after resveratrol treatment and then 
      remained high 36 h later. Meanwhile, the production of GDNF was initially 
      increased by up to four-fold 24 h after resveratrol treatment and continued to 
      increase to six-fold at 36 h and remained at a high level till 48 h. Western blot 
      analysis of BDNF and GDNF protein in astroglia at different time points after 
      resveratrol treatment indicated similar increases. Furthermore, resveratrol 
      significantly induced the phosphorylation of extracellular signal-regulated 
      kinase 1/2 (ERK1/2) and cAMP responsive element-binding protein (CREB) in 
      astroglia. Overall, resveratrol is effective in promoting astroglia-derived 
      neurotrophic factor release, and this effect is mediated, at least in part, by 
      the activation of ERK1/2 and CREB.
FAU - Zhang, Feng
AU  - Zhang F
AD  - Department of Pharmacology and Key Lab of Basic Pharmacology of Guizhou, Zunyi 
      Medical College, Zunyi, People's Republic of China. zhangfengzmc@163.com
FAU - Lu, Yuan-Fu
AU  - Lu YF
FAU - Wu, Qin
AU  - Wu Q
FAU - Liu, Jie
AU  - Liu J
FAU - Shi, Jing-Shan
AU  - Shi JS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120808
PL  - Switzerland
TA  - Exp Biol Med (Maywood)
JT  - Experimental biology and medicine (Maywood, N.J.)
JID - 100973463
RN  - 0 (Culture Media)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (Stilbenes)
RN  - Q369O8926L (Resveratrol)
SB  - IM
MH  - Animals
MH  - Astrocytes/*drug effects/*metabolism
MH  - Blotting, Western
MH  - Cells, Cultured
MH  - Culture Media/chemistry
MH  - Female
MH  - Nerve Growth Factors/*metabolism
MH  - Neuroprotective Agents/*metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Resveratrol
MH  - Stilbenes/*metabolism
MH  - Time Factors
EDAT- 2012/08/10 06:00
MHDA- 2012/11/01 06:00
CRDT- 2012/08/10 06:00
PHST- 2012/08/10 06:00 [entrez]
PHST- 2012/08/10 06:00 [pubmed]
PHST- 2012/11/01 06:00 [medline]
AID - ebm.2012.012044 [pii]
AID - 10.1258/ebm.2012.012044 [doi]
PST - ppublish
SO  - Exp Biol Med (Maywood). 2012 Aug;237(8):943-8. doi: 10.1258/ebm.2012.012044. Epub 
      2012 Aug 8.