PMID- 2287770
OWN - NLM
STAT- MEDLINE
DCOM- 19910326
LR  - 20190515
IS  - 0886-022X (Print)
IS  - 0886-022X (Linking)
VI  - 12
IP  - 3
DP  - 1990
TI  - Adenylate cyclase responses and biochemical characterization of primary rabbit 
      proximal tubular cell cultures and LLC-PK1 cells.
PG  - 177-82
AB  - Freshly isolated rabbit proximal tubules (PT), confluent primary rabbit proximal 
      tubule cultures (PTC) and LLC-PK1 cells were characterised. Brushborder enzyme 
      activities were lower in PTC than in LLC-PK1: ratios were 0.026 for alkaline 
      phosphatase (AP), 0.458 for alanine aminopeptidase (AAP) and 0.514 for 
      gamma-glutamyl transpeptidase (GGT). PT/PTC ratios were 79.7 for AP, 7.96 for AAP 
      and 3.45 for GGT. Specific activities of hexokinase (HK) and lactate 
      dehydrogenase (LDH) were high in cultured cells as compared to PT: PT/PTC ratios 
      were 0.063 and 0.033, while PTC/LLC-PK1 ratios were 0.406 and 1.19 for HK and LDH 
      respectively. PTC/LLC-PK1 ratios were 2.21 for Na/K ATPase, 2.07 for succinate 
      dehydrogenase, 1.12 for cathepsin B, 0.607 for N-acetyl-beta-D-glucosaminidase 
      and 8.98 for glutathione-S-transferase. Adenylate cyclase response to 
      parathormone (PTH), was similar in PTC and PT, but stimulated/basal ratios were 
      higher in PT than in PTC. LLC-PK1 cells were stimulated by thyrocalcitonin (SCT), 
      arginin-vasopressin (AVP) and PTH; stimulated/basal ratios ranked AVP greater 
      than PTH greater than SCT. Differences between both types of cultures affect the 
      choice of in vitro model for nephrotoxicity studies.
FAU - Toutain, H
AU  - Toutain H
AD  - INSERM U-295, UER Medecine-Pharmacie de Rouen, Saint Etienne du Rouvray, France.
FAU - Courjault, F
AU  - Courjault F
FAU - Vauclin-Jacques, N
AU  - Vauclin-Jacques N
FAU - Morin, J P
AU  - Morin JP
LA  - eng
PT  - Journal Article
PL  - England
TA  - Ren Fail
JT  - Renal failure
JID - 8701128
RN  - 0 (Culture Media)
RN  - EC 4.6.1.1 (Adenylyl Cyclases)
SB  - IM
MH  - Adenylyl Cyclases/*metabolism
MH  - Animals
MH  - Cell Line
MH  - Cell Membrane/enzymology
MH  - Cells, Cultured
MH  - Culture Media
MH  - Kidney Tubules, Proximal/cytology/*enzymology
MH  - Lysosomes/enzymology
MH  - Microvilli/enzymology
MH  - Rabbits
EDAT- 1990/01/01 00:00
MHDA- 1990/01/01 00:01
CRDT- 1990/01/01 00:00
PHST- 1990/01/01 00:00 [pubmed]
PHST- 1990/01/01 00:01 [medline]
PHST- 1990/01/01 00:00 [entrez]
AID - 10.3109/08860229009065561 [doi]
PST - ppublish
SO  - Ren Fail. 1990;12(3):177-82. doi: 10.3109/08860229009065561.