PMID- 22877943
OWN - NLM
STAT- MEDLINE
DCOM- 20130301
LR  - 20201219
IS  - 1472-6491 (Electronic)
IS  - 1472-6483 (Linking)
VI  - 25
IP  - 4
DP  - 2012 Oct
TI  - Evidence that miR-133a causes recurrent spontaneous abortion by reducing HLA-G 
      expression.
PG  - 415-24
LID - 10.1016/j.rbmo.2012.06.022 [doi]
AB  - Human leukocyte antigen (HLA)-G is thought to confer fetal-maternal tolerance and 
      play a crucial role in ensuring a successful pregnancy. There is increasing 
      evidence that HLA-G is regulated at the post-transcriptional level. This study 
      investigated the role of miR-133a in regulating HLA-G expression and the 
      pathogenesis of recurrent spontaneous abortion (RSA). Twelve patients (25-30 
      years) with RSA at 7 gestational weeks were screened by array-based comparative 
      genome hybridization: 16.7% were found to have an abnormal karyotype and all 
      induced abortion (IA) patients had normal karyotype. The villi of RSA and IA 
      patients with normal karyotype were further screened by miRNA microarrays. 
      Multi-software prediction and real-time PCR confirmed that miR-133a was most 
      likely to bind to HLA-G 3' untranscribed region (UTR). Relevance analysis showed 
      that, compared with IA villi, miR-133a was greatly overexpressed in RSA villi 
      with normal karyotype (P<0.01), but not in abnormal RSA villi. A luciferase 
      reporter assay suggested that miR-133a interacted with HLA-G 3' UTR. 
      Overexpression of miR-133a in JEG-3 cells decreased HLA-G expression at the 
      protein level, with no effect on mRNA. These findings provide strong evidence 
      that miR-133a regulates HLA-G expression by reducing translation and is involved 
      in the pathogenesis of RSA.
CI  - Copyright (c) 2012 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All 
      rights reserved.
FAU - Wang, Xiaohong
AU  - Wang X
AD  - Department of Obstetrics and Gynecology, Tangdu Hospital, The Fourth Military 
      Medical University, Xi'an 710038, China. xhwang_1968@126.com
FAU - Li, Bo
AU  - Li B
FAU - Wang, Jun
AU  - Wang J
FAU - Lei, Jie
AU  - Lei J
FAU - Liu, Chuang
AU  - Liu C
FAU - Ma, Yuan
AU  - Ma Y
FAU - Zhao, Hongxi
AU  - Zhao H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120720
PL  - Netherlands
TA  - Reprod Biomed Online
JT  - Reproductive biomedicine online
JID - 101122473
RN  - 0 (3' Untranslated Regions)
RN  - 0 (HLA-G Antigens)
RN  - 0 (MIRN133 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Mutant Proteins)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - 3' Untranslated Regions
MH  - Abortion, Habitual/genetics/*metabolism
MH  - Adult
MH  - Cell Line
MH  - China
MH  - Chorionic Villi/*metabolism
MH  - Chorionic Villi Sampling
MH  - Comparative Genomic Hybridization
MH  - *Down-Regulation
MH  - Female
MH  - Genes, Reporter
MH  - HLA-G Antigens/genetics/*metabolism
MH  - Humans
MH  - Karyotype
MH  - MicroRNAs/genetics/*metabolism
MH  - Mutant Proteins/metabolism
MH  - Pregnancy
MH  - Pregnancy Trimester, First
MH  - Recombinant Proteins/metabolism
MH  - *Up-Regulation
EDAT- 2012/08/11 06:00
MHDA- 2013/03/02 06:00
CRDT- 2012/08/11 06:00
PHST- 2012/02/03 00:00 [received]
PHST- 2012/06/16 00:00 [revised]
PHST- 2012/06/21 00:00 [accepted]
PHST- 2012/08/11 06:00 [entrez]
PHST- 2012/08/11 06:00 [pubmed]
PHST- 2013/03/02 06:00 [medline]
AID - S1472-6483(12)00418-X [pii]
AID - 10.1016/j.rbmo.2012.06.022 [doi]
PST - ppublish
SO  - Reprod Biomed Online. 2012 Oct;25(4):415-24. doi: 10.1016/j.rbmo.2012.06.022. 
      Epub 2012 Jul 20.