PMID- 22878185
OWN - NLM
STAT- MEDLINE
DCOM- 20130204
LR  - 20240109
IS  - 1347-6947 (Electronic)
IS  - 0916-8451 (Linking)
VI  - 76
IP  - 8
DP  - 2012
TI  - Dieckol isolated from Ecklonia cava protects against high-glucose induced damage 
      to rat insulinoma cells by reducing oxidative stress and apoptosis.
PG  - 1445-51
AB  - Pancreatic beta cells are very sensitive to oxidative stress and this might play an 
      important role in beta cell death with diabetes. The protective effect of dieckol, 
      one of the phlorotannin polyphenol compounds purified from Ecklonia cava (E. 
      cava), against high glucose-induced oxidative stress was investigated by using 
      rat insulinoma cells. A high-glucose (30 mM) treatment induced the death of rat 
      insulinoma cells, but dieckol, at a concentration 17.5 or 70 microM, significantly 
      inhibited the high-glucose induced glucotoxicity. Treatment with dieckol also 
      dose-dependently reduced thiobarbituric acid reactive substances (TBARS), the 
      generation of intracellular reactive oxygen species (ROS), and the nitric oxide 
      level increased by a high glucose concentration. In addition, the dieckol 
      treatment increased the activities of antioxidative enzymes including catalase 
      (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-px) in high 
      glucose-pretreated rat insulinoma cells. Dieckol protected rat insulinoma cells 
      damage under high glucose conditions. These effects were mediated by suppressing 
      apoptosis and were associated with increased anti-apoptotic Bcl-2 expression, and 
      reduced pro-apoptotic cleaved caspase-3 expression. These findings indicate that 
      dieckol might be useful as a potential pharmaceutical agent to protect against 
      the glucotoxicity caused by hyperglycemia-induced oxidative stress associated 
      with diabetes.
FAU - Lee, Seung-Hong
AU  - Lee SH
AD  - Department of Marine Life Science, Jeju National University, Jeju 690-756, Korea.
FAU - Park, Mi-Hwa
AU  - Park MH
FAU - Kang, Sung-Myung
AU  - Kang SM
FAU - Ko, Seok-Chun
AU  - Ko SC
FAU - Kang, Min-Cheol
AU  - Kang MC
FAU - Cho, Seungmok
AU  - Cho S
FAU - Park, Pyo-Jam
AU  - Park PJ
FAU - Jeon, Byong-Tae
AU  - Jeon BT
FAU - Kim, Se-Kwon
AU  - Kim SK
FAU - Han, Ji-Sook
AU  - Han JS
FAU - Jeon, You-Jin
AU  - Jeon YJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120807
PL  - England
TA  - Biosci Biotechnol Biochem
JT  - Bioscience, biotechnology, and biochemistry
JID - 9205717
RN  - 0 (Antioxidants)
RN  - 0 (Benzofurans)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Thiobarbituric Acid Reactive Substances)
RN  - 0 (dieckol)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 3.4.22.- (Caspase 3)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Antioxidants/isolation & purification/*pharmacology
MH  - Apoptosis/drug effects
MH  - Benzofurans/isolation & purification/*pharmacology
MH  - Caspase 3/genetics/metabolism
MH  - Catalase/genetics/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Gene Expression/drug effects
MH  - Glucose/adverse effects
MH  - Glutathione Peroxidase/genetics/metabolism
MH  - Insulinoma/metabolism/pathology
MH  - Nitric Oxide/antagonists & inhibitors/biosynthesis
MH  - Oxidative Stress/drug effects
MH  - Phaeophyceae/*chemistry
MH  - Proto-Oncogene Proteins c-bcl-2/genetics/metabolism
MH  - Rats
MH  - Reactive Oxygen Species/*antagonists & inhibitors/metabolism
MH  - Superoxide Dismutase/genetics/metabolism
MH  - Thiobarbituric Acid Reactive Substances/metabolism
MH  - Tumor Cells, Cultured
EDAT- 2012/08/11 06:00
MHDA- 2013/02/05 06:00
CRDT- 2012/08/11 06:00
PHST- 2012/08/11 06:00 [entrez]
PHST- 2012/08/11 06:00 [pubmed]
PHST- 2013/02/05 06:00 [medline]
AID - DN/JST.JSTAGE/bbb/120096 [pii]
AID - 10.1271/bbb.120096 [doi]
PST - ppublish
SO  - Biosci Biotechnol Biochem. 2012;76(8):1445-51. doi: 10.1271/bbb.120096. Epub 2012 
      Aug 7.