PMID- 22882187
OWN - NLM
STAT- MEDLINE
DCOM- 20130909
LR  - 20181202
IS  - 1574-6941 (Electronic)
IS  - 0168-6496 (Linking)
VI  - 83
IP  - 1
DP  - 2013 Jan
TI  - Anaerobic oxidation of methane in hypersaline cold seep sediments.
PG  - 214-31
LID - 10.1111/j.1574-6941.2012.01466.x [doi]
AB  - Life in hypersaline environments is typically limited by bioenergetic 
      constraints. Microbial activity at the thermodynamic edge, such as the anaerobic 
      oxidation of methane (AOM) coupled to sulphate reduction (SR), is thus unlikely 
      to thrive in these environments. In this study, carbon and sulphur cycling was 
      investigated in the extremely hypersaline cold seep sediments of Mercator mud 
      volcano. AOM activity was partially inhibited but still present at salinity 
      levels of 292 g L(-1) (c. eightfold sea water concentration) with rates of 2.3 
      nmol cm(-3) day(-1) and was even detectable under saturated conditions. Methane 
      and evaporite-derived sulphate comigrated in the ascending geofluids, which, in 
      combination with a partial activity inhibition, resulted in AOM activity being 
      spread over unusually wide depth intervals. Up to 79% of total cells in the AOM 
      zone were identified by fluorescence in situ hybridization (FISH) as anaerobic 
      methanotrophs of the ANME-1. Most ANME-1 cells formed monospecific chains without 
      any attached partner. At all sites, AOM activity co-occurred with SR activity and 
      sometimes significantly exceeded it. Possible causes of these unexpected results 
      are discussed. This study demonstrates that in spite of a very low energy yield 
      of AOM, microorganisms carrying this reaction can thrive in salinity up to halite 
      saturation.
CI  - (c) 2012 Federation of European Microbiological Societies. Published by Blackwell 
      Publishing Ltd. All rights reserved.
FAU - Maignien, Lois
AU  - Maignien L
AD  - Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Ghent, 
      Belgium. lmaignien@mbl.edu
FAU - Parkes, R John
AU  - Parkes RJ
FAU - Cragg, Barry
AU  - Cragg B
FAU - Niemann, Helge
AU  - Niemann H
FAU - Knittel, Katrin
AU  - Knittel K
FAU - Coulon, Stephanie
AU  - Coulon S
FAU - Akhmetzhanov, Andrey
AU  - Akhmetzhanov A
FAU - Boon, Nico
AU  - Boon N
LA  - eng
PT  - Journal Article
DEP - 20121017
PL  - England
TA  - FEMS Microbiol Ecol
JT  - FEMS microbiology ecology
JID - 8901229
RN  - 0 (DNA, Archaeal)
RN  - 0 (DNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sulfates)
RN  - OP0UW79H66 (Methane)
SB  - IM
MH  - Anaerobiosis
MH  - Archaea/classification/isolation & purification/metabolism
MH  - Bacteria/classification/isolation & purification/metabolism
MH  - DNA, Archaeal/genetics/isolation & purification
MH  - DNA, Bacterial/genetics/isolation & purification
MH  - Geologic Sediments/*microbiology
MH  - Methane/*metabolism
MH  - Oxidation-Reduction
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Salinity
MH  - Seawater/chemistry/*microbiology
MH  - Sulfates/metabolism
MH  - *Water Microbiology
EDAT- 2012/08/14 06:00
MHDA- 2013/09/10 06:00
CRDT- 2012/08/14 06:00
PHST- 2012/04/20 00:00 [received]
PHST- 2012/07/28 00:00 [revised]
PHST- 2012/08/01 00:00 [accepted]
PHST- 2012/08/14 06:00 [entrez]
PHST- 2012/08/14 06:00 [pubmed]
PHST- 2013/09/10 06:00 [medline]
AID - 10.1111/j.1574-6941.2012.01466.x [doi]
PST - ppublish
SO  - FEMS Microbiol Ecol. 2013 Jan;83(1):214-31. doi: 
      10.1111/j.1574-6941.2012.01466.x. Epub 2012 Oct 17.