PMID- 22882594
OWN - NLM
STAT- MEDLINE
DCOM- 20130418
LR  - 20121030
IS  - 1365-2559 (Electronic)
IS  - 0309-0167 (Linking)
VI  - 61
IP  - 5
DP  - 2012 Nov
TI  - Fluorescence in situ hybridization for the differential diagnosis between Spitz 
      naevus and spitzoid melanoma.
PG  - 899-909
LID - 10.1111/j.1365-2559.2012.04293.x [doi]
AB  - AIMS: The differential diagnosis between Spitz naevus and spitzoid melanoma can 
      be extremely difficult, or even impossible. In recent years, many attempts have 
      been made to find specific histopathological or immunohistochemical markers, 
      although none has proved successful. Because the prognosis and treatment of each 
      are very different, it is important to distinguish between these entities. We 
      evaluated the ability of the fluorescence in situ hybridization (FISH) 
      assay-designed to detect the copy number of the RREB1 (6p25), MYB (6q23) and 
      CCND1 (11q13) genes and of centromere 6 (Cep 6)-in order to distinguish between 
      Spitz naevus and spitzoid melanoma. METHODS AND RESULTS: We evaluated 12 spitzoid 
      melanomas and six Spitz naevi from our records. The diagnosis of both conditions 
      was based on previously described histopathological criteria. We obtained 
      valuable results for FISH in eight spitzoid melanomas and five Spitz naevi. 
      Chromosomal aberrations were detected in seven of the eight spitzoid melanomas 
      (FISH-positive) and in none of the five Spitz naevi. The FISH-negative spitzoid 
      melanoma was the least typical in its group. CONCLUSIONS: FISH was able to 
      distinguish between Spitz naevus and spitzoid melanoma, with a sensitivity of 
      87.5% and a specificity of 100%. Our findings suggest that FISH could prove a 
      useful tool in the differential diagnosis between these entities.
CI  - (c) 2012 Blackwell Publishing Limited.
FAU - Requena, Celia
AU  - Requena C
AD  - Department of Dermatology, Instituto Valenciano de Oncologia, Valencia, Spain.
FAU - Rubio, Luis
AU  - Rubio L
FAU - Traves, Victor
AU  - Traves V
FAU - Sanmartin, Onofre
AU  - Sanmartin O
FAU - Nagore, Eduardo
AU  - Nagore E
FAU - Llombart, Beatriz
AU  - Llombart B
FAU - Serra, Carlos
AU  - Serra C
FAU - Fernandez-Serra, Antonio
AU  - Fernandez-Serra A
FAU - Botella, Rafael
AU  - Botella R
FAU - Guillen, Carlos
AU  - Guillen C
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120808
PL  - England
TA  - Histopathology
JT  - Histopathology
JID - 7704136
RN  - 0 (CCND1 protein, human)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (RREB1 protein, human)
RN  - 0 (Transcription Factors)
RN  - 136601-57-5 (Cyclin D1)
SB  - IM
MH  - Adult
MH  - Child
MH  - Chromosome Aberrations
MH  - Cyclin D1/genetics
MH  - DNA-Binding Proteins/genetics
MH  - Diagnosis, Differential
MH  - Female
MH  - Gene Dosage
MH  - Genes, myb
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Melanoma/*diagnosis/*genetics/pathology
MH  - Middle Aged
MH  - Nevus, Epithelioid and Spindle Cell/*diagnosis/*genetics/pathology
MH  - Skin Neoplasms/*diagnosis/*genetics/pathology
MH  - Transcription Factors/genetics
MH  - Young Adult
EDAT- 2012/08/14 06:00
MHDA- 2013/04/20 06:00
CRDT- 2012/08/14 06:00
PHST- 2012/08/14 06:00 [entrez]
PHST- 2012/08/14 06:00 [pubmed]
PHST- 2013/04/20 06:00 [medline]
AID - 10.1111/j.1365-2559.2012.04293.x [doi]
PST - ppublish
SO  - Histopathology. 2012 Nov;61(5):899-909. doi: 10.1111/j.1365-2559.2012.04293.x. 
      Epub 2012 Aug 8.