PMID- 22883001
OWN - NLM
STAT- MEDLINE
DCOM- 20130320
LR  - 20221207
IS  - 0376-2491 (Print)
IS  - 0376-2491 (Linking)
VI  - 92
IP  - 17
DP  - 2012 May 8
TI  - [Association between amino acid mutations in Gag protein and human leukocyte 
      antigen class I alleles in human immunodeficiency virus-1 B' infected Han Chinese 
      people].
PG  - 1159-64
AB  - OBJECTIVE: To explore the human leukocyte antigen (HLA)-associated mutations in 
      Gag protein of B' clade (human immunodeficiency virus-1) HIV-1 infected Han 
      Chinese people and evaluate the impact of HLA associated Gag mutations on the 
      disease progression of HIV infection. METHODS: A total of 95 B' clade HIV-1 
      infected Han Chinese cases were recruited. The gag sequences were amplified from 
      viral RNA and sequenced directly. HLA-I genotypes were detected with the assay of 
      polymerase chain reaction-sequence specific primer (PCR-SSP). HLA-associated 
      mutations were identified and the relationships between HLA-associated mutations 
      and CD4+ T cell counts or plasma viral loads analyzed. RESULTS: Forty-seven kinds 
      of mutations at 28 sites (15, 18, 26, 30, 34, 46, 62, 67, 81, 84, 90, 102, 118, 
      121, 122, 125, 146, 147, 173, 176, 252, 357, 374, 376, 437, 470, 471, 478) of Gag 
      protein were significantly associated with particular HLA class I allelotypes (P 
      < 0.05). Among which, 9 sites (26, 30, 81, 84, 125, 146, 147, 357, 437) were 
      located within 13 known cytotoxic T-lymphocyte (CTL) epitopes or flanking 
      regions. The number of HLA-associated mutations was significantly associated with 
      both CD4 T cell counts (r = -0.318, P = 0.002) and viral loads (r = 0.360, P = 
      0.003). CONCLUSION: HLA-associated mutations may have a significant impact on HIV 
      disease progression in B' clade HIV-1 infected Han Chinese population.
FAU - Han, Xiao-xu
AU  - Han XX
AD  - Key Laboratory of AIDS Immunology of Ministry of Health, Department of Laboratory 
      Medicine, First Hospital, China Medical University, Shenyang 110001, China.
FAU - Zhang, Hui
AU  - Zhang H
FAU - Zhao, Bin
AU  - Zhao B
FAU - Chen, Ou
AU  - Chen O
FAU - Zhang, Cheng
AU  - Zhang C
FAU - Ji, Yang-tao
AU  - Ji YT
FAU - Zhang, Zi-ning
AU  - Zhang ZN
FAU - Cui, Wei-guo
AU  - Cui WG
FAU - Liu, Bao-gui
AU  - Liu BG
FAU - Duan, Song
AU  - Duan S
FAU - Shang, Hong
AU  - Shang H
LA  - chi
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - China
TA  - Zhonghua Yi Xue Za Zhi
JT  - Zhonghua yi xue za zhi
JID - 7511141
RN  - 0 (RNA, Viral)
RN  - 0 (gag Gene Products, Human Immunodeficiency Virus)
SB  - IM
MH  - Acquired Immunodeficiency Syndrome/*genetics/*virology
MH  - Adult
MH  - Aged
MH  - Alleles
MH  - Asian People/genetics
MH  - Female
MH  - *Genes, MHC Class I
MH  - HIV-1/*genetics
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Mutation
MH  - RNA, Viral
MH  - Viral Load
MH  - Young Adult
MH  - gag Gene Products, Human Immunodeficiency Virus/*genetics
EDAT- 2012/08/14 06:00
MHDA- 2013/03/21 06:00
CRDT- 2012/08/14 06:00
PHST- 2012/08/14 06:00 [entrez]
PHST- 2012/08/14 06:00 [pubmed]
PHST- 2013/03/21 06:00 [medline]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi. 2012 May 8;92(17):1159-64.