PMID- 22883750
OWN - NLM
STAT- MEDLINE
DCOM- 20130128
LR  - 20191210
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 39
IP  - 1
DP  - 2013 Jan 15
TI  - Sensitive label-free electrochemical analysis of human IgE using an aptasensor 
      with cDNA amplification.
PG  - 133-8
LID - 10.1016/j.bios.2012.07.009 [doi]
AB  - In this study, we developed an ultrasensitive label-free aptamer-based 
      electrochemical biosensor, featuring a highly specific anti-human immunoglobulin 
      E (IgE) aptamer as a capture probe, for human IgE detection. Construction of the 
      aptasensor began with the electrodeposition of gold nanoparticles (AuNPs) onto a 
      graphite-based screen-printed electrode (SPE). After immobilizing the 
      thiol-capped anti-human IgE aptamer onto the AuNPs through self-assembly, we 
      treated the electrode with mercaptohexanol (MCH) to ensure that the remaining 
      unoccupied surfaces of the AuNPs would not undergo nonspecific binding. We 
      employed a designed complementary DNA featuring a guanine-rich section in its 
      sequence (cDNA G1) as a detection probe to bind with the unbound anti-human IgE 
      aptamer. We measured the redox current of methylene blue (MB) to determine the 
      concentration of human IgE in the sample. When the aptamer captured human IgE, 
      the binding of cDNA G1 to the aptamer was inhibited. Using cDNA G1 in the assay 
      greatly amplified the redox signal of MB bound to the detection probe. 
      Accordingly, this approach allowed the linear range (coefficient of 
      determination: 0.996) for the analysis of human IgE to extend from 1 to 
      100,000pM; the limit of detection was 0.16pM. The fabricated aptasensor exhibited 
      good selectivity toward human IgE even when human IgG, thrombin, and human serum 
      albumin were present at 100-fold concentrations. This method should be readily 
      applicable to the detection of other analytes, merely by replacing the anti-human 
      IgE aptamer/cDNA G1 pair with a suitable anti-target molecule aptamer and cDNA.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Lee, Cheng-Yu
AU  - Lee CY
AD  - Department of Applied Chemistry, National Chiao Tung University, Hsinchu, Taiwan, 
      ROC.
FAU - Wu, Kuan-Ying
AU  - Wu KY
FAU - Su, Hsiu-Li
AU  - Su HL
FAU - Hung, Huan-Yi
AU  - Hung HY
FAU - Hsieh, You-Zung
AU  - Hsieh YZ
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120724
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 0 (Aptamers, Nucleotide)
RN  - 0 (DNA, Complementary)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 7440-57-5 (Gold)
RN  - EC 3.4.21.5 (Thrombin)
SB  - IM
MH  - Aptamers, Nucleotide/*chemistry
MH  - Base Sequence
MH  - Biosensing Techniques/methods
MH  - DNA, Complementary/chemistry
MH  - Electrochemical Techniques/*methods
MH  - Electrodes
MH  - Gold/chemistry
MH  - Humans
MH  - Immunoglobulin E/*analysis
MH  - Limit of Detection
MH  - Metal Nanoparticles/chemistry
MH  - Thrombin/analysis
EDAT- 2012/08/14 06:00
MHDA- 2013/01/29 06:00
CRDT- 2012/08/14 06:00
PHST- 2012/04/26 00:00 [received]
PHST- 2012/07/06 00:00 [revised]
PHST- 2012/07/07 00:00 [accepted]
PHST- 2012/08/14 06:00 [entrez]
PHST- 2012/08/14 06:00 [pubmed]
PHST- 2013/01/29 06:00 [medline]
AID - S0956-5663(12)00429-0 [pii]
AID - 10.1016/j.bios.2012.07.009 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2013 Jan 15;39(1):133-8. doi: 10.1016/j.bios.2012.07.009. 
      Epub 2012 Jul 24.