PMID- 22884798
OWN - NLM
STAT- MEDLINE
DCOM- 20130118
LR  - 20120910
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 425
IP  - 4
DP  - 2012 Sep 7
TI  - miR-142-3p is essential for hematopoiesis and affects cardiac cell fate in 
      zebrafish.
PG  - 755-61
LID - 10.1016/j.bbrc.2012.07.148 [doi]
AB  - MicroRNAs (miRNAs) play a pivotal role during embryonic development and are 
      required for proper organogenesis, including hematopoiesis. Recent studies 
      suggest that, in the early mesoderm, there is an interaction between the 
      hematopoietic and cardiac lineages. However, whether miRNAs can affect other 
      lineages remains unknown. Therefore, we investigated whether hematopoietic 
      miR-142-3p modulated the mesoderm formation. We report that knockdown (KD) of 
      miR-142-3p, a hematopoietic-specific miRNA, in zebrafish resulted in loss of 
      hematopoiesis during embryonic development. Intriguingly, we observed abnormal 
      cardiac phenotypes and insufficiency of somitegenesis in KD-morphants. In the 
      early developmental stage, a tiny heart, contractile dysfunction in the 
      ventricle, cardiac arrhythmia (e.g. a 2:1 ratio of atrial:ventricular beating), 
      and bradycardia were consistently observed. Histological examination revealed 
      severe hypoplasia of the ventricle and disrupted muscle alignment. To determine 
      the mechanism, we performed DNA microarray analysis. The results revealed that 
      the expression of several mesodermal genes essential for the formation of cardiac 
      and somatic mesoderm, such as no tail, T-box gene 16, mesoderm posterior a, one 
      eye pinhead, and rho-associated, coiled-coil containing protein kinase (Rock2a), 
      were increased in miR-142-3p KD-morphants. The luciferase reporter assay revealed 
      that miR-142-3p repressed luciferase activity on the Rock2a 3'-UTR. The findings 
      of the present study indicate that miR-142-3p plays a critical role in 
      hematopoiesis, cardiogenesis, and somitegenesis in the early stage of mesoderm 
      formation via regulation of Rock2a.
CI  - Copyright (c) 2012 Elsevier Inc. All rights reserved.
FAU - Nishiyama, Takahiko
AU  - Nishiyama T
AD  - Department of Cardiology, Keio University School of Medicine, Tokyo 160-8582, 
      Japan.
FAU - Kaneda, Ruri
AU  - Kaneda R
FAU - Ono, Tomohiko
AU  - Ono T
FAU - Tohyama, Shugo
AU  - Tohyama S
FAU - Hashimoto, Hisayuki
AU  - Hashimoto H
FAU - Endo, Jin
AU  - Endo J
FAU - Tsuruta, Hikaru
AU  - Tsuruta H
FAU - Yuasa, Shinsuke
AU  - Yuasa S
FAU - Ieda, Masaki
AU  - Ieda M
FAU - Makino, Shinji
AU  - Makino S
FAU - Fukuda, Keiichi
AU  - Fukuda K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120802
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (MIRN142 microRNA, zebrafish)
RN  - 0 (MicroRNAs)
RN  - EC 1.13.12.- (Luciferases)
SB  - IM
MH  - Animals
MH  - Gene Expression Regulation, Developmental
MH  - Gene Knockdown Techniques
MH  - Genes, Reporter
MH  - Heart/*embryology/physiology
MH  - *Hematopoiesis
MH  - Luciferases/biosynthesis/genetics
MH  - MicroRNAs/genetics/*metabolism
MH  - *Organogenesis
MH  - Zebrafish/*embryology/genetics/metabolism
EDAT- 2012/08/14 06:00
MHDA- 2013/01/19 06:00
CRDT- 2012/08/14 06:00
PHST- 2012/07/20 00:00 [received]
PHST- 2012/07/26 00:00 [accepted]
PHST- 2012/08/14 06:00 [entrez]
PHST- 2012/08/14 06:00 [pubmed]
PHST- 2013/01/19 06:00 [medline]
AID - S0006-291X(12)01462-3 [pii]
AID - 10.1016/j.bbrc.2012.07.148 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2012 Sep 7;425(4):755-61. doi: 
      10.1016/j.bbrc.2012.07.148. Epub 2012 Aug 2.