PMID- 22885051
OWN - NLM
STAT- MEDLINE
DCOM- 20121203
LR  - 20120827
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1256
DP  - 2012 Sep 21
TI  - High polar organic-inorganic hybrid coating stir bar sorptive extraction combined 
      with high performance liquid chromatography-inductively coupled plasma mass 
      spectrometry for the speciation of seleno-amino acids and seleno-oligopeptides in 
      biological samples.
PG  - 32-9
LID - 10.1016/j.chroma.2012.07.080 [doi]
AB  - In this work, partially sulfonated polystyrene-titania (PSP-TiO(2)) 
      organic-inorganic hybrid stir bar coating was prepared by sol-gel and blending 
      methods, and a new method of PSP-TiO(2) coating stir bar sorptive extraction 
      (SBSE)-high performance liquid chromatography (HPLC)-inductively coupled plasma 
      mass spectrometry (ICP-MS) was established for the analysis of seleno-amino acids 
      (selenocystine (SeCys(2)), methylseleno-cysteine (MeSeCys), selenomethionine 
      (SeMet) and selenoethionine (SeEt)) and seleno-oligopeptides 
      (gamma-glutamyl-Se-methyl-selenocysteine (gamma-GluMeSeCys) and selenodiglutathione 
      (GS-Se-SG)) in biological samples. The prepared high polar PSP-TiO(2) hybrid 
      coating avoided the swelling of PSP and cracking of TiO(2) coating by combining 
      the good film-forming property of PSP with the high mechanical strength of 
      TiO(2). The scanning electron microscope (SEM) showed that no obvious swelling 
      and damage occurred for the PSP-TiO(2) hybrid stir bar coating after 30 
      extraction/desorption cycles. The preparation reproducibility of PSP-TiO(2) 
      coated stir bar, evaluated with the relative standard deviations (RSDs), was in 
      the range of 6.7-12.6% (n=5) in one batch, and 9.9-17.6% (n=7) among different 
      batches. The limits of detection (LODs) of the developed method for six target 
      selenium species were in the range of 50.2-185.5 ngL(-1) (as (77)Se) and 
      45.9-158.8 ngL(-1) (as (82)Se) with the RSDs within 4.9-11.7%. The dynamic linear 
      range was found to cover three orders of magnitude with correlation coefficient 
      of 0.9995-0.9999. The developed method was applied for the analysis of Certified 
      Reference Material SELM-1 selenium enriched yeast and the determined values were 
      in good agreement with the certified values. The method has also been applied for 
      the analysis of seleno-amino acids and seleno-oligopeptides in human urine and 
      garlic samples. Different from the conventional organic polymer SBSE coatings 
      (such as polydimethylsiloxane, PDMS), the extraction mechanism of PSP-TiO(2) 
      organic-inorganic hybrid SBSE coating was based on the cation exchange 
      interaction, which made it feasible to directly extract high polar seleno-amino 
      acids and seleno-oligopeptides in biological samples without derivatization. This 
      coating could also be suitable for stir bar sorptive extraction of other cationic 
      compounds from the environmental and biological samples.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Mao, Xiangju
AU  - Mao X
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan 430072, China.
FAU - Hu, Bin
AU  - Hu B
FAU - He, Man
AU  - He M
FAU - Chen, Beibei
AU  - Chen B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120801
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Oligopeptides)
RN  - 0 (Selenium Compounds)
SB  - IM
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Hydrogen-Ion Concentration
MH  - Limit of Detection
MH  - Mass Spectrometry/*methods
MH  - Microscopy, Electron, Scanning
MH  - Oligopeptides/*chemistry
MH  - Reproducibility of Results
MH  - Selenium Compounds/*chemistry
EDAT- 2012/08/14 06:00
MHDA- 2012/12/10 06:00
CRDT- 2012/08/14 06:00
PHST- 2012/06/15 00:00 [received]
PHST- 2012/07/26 00:00 [revised]
PHST- 2012/07/26 00:00 [accepted]
PHST- 2012/08/14 06:00 [entrez]
PHST- 2012/08/14 06:00 [pubmed]
PHST- 2012/12/10 06:00 [medline]
AID - S0021-9673(12)01153-3 [pii]
AID - 10.1016/j.chroma.2012.07.080 [doi]
PST - ppublish
SO  - J Chromatogr A. 2012 Sep 21;1256:32-9. doi: 10.1016/j.chroma.2012.07.080. Epub 
      2012 Aug 1.