PMID- 22885052
OWN - NLM
STAT- MEDLINE
DCOM- 20121203
LR  - 20120827
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1256
DP  - 2012 Sep 21
TI  - On-line derivatization with on-line coupled normal phase liquid 
      chromatography-gas chromatography using the through oven transfer adsorption 
      desorption interface: application to the analysis of total sterols in edible 
      oils.
PG  - 191-6
LID - 10.1016/j.chroma.2012.07.057 [doi]
AB  - In the present work on-line derivatization is combined with on-line normal phase 
      liquid chromatography-gas chromatography (NPLC-GC) to analyze total sterols in 
      edible oils. The method uses the TOTAD interface with an additional LC injection 
      valve to automatically introduce the derivatization reagent. The derivatization 
      reaction takes place in the adsorbent material located inside the liner of the 
      TOTAD interface. The samples were saponified with potassium hydroxide in an 
      ethanolic solution and the unsaponifiable fraction was extracted with diethyl 
      ether. The extract was then analyzed by on-line derivatization with on-line 
      NPLC-GC, avoiding the laborious thin layer chromatography and off-line 
      derivatization steps used in the Official European Union (EU) method. The 
      relative standard deviations (RSDs) from the absolute peak area were lower than 
      14% except for campestanol. No variability in retention time was observed. Limits 
      of detection (LODs) were less than 8.82 mg/kg. Different edible oils were 
      analyzed using the proposed method and the results obtained were compared with 
      those obtained using the Official EU method. There was good agreement between 
      both methods for all the sterols analyzed.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Toledano, Rosa M
AU  - Toledano RM
AD  - Escuela Tecnica Superior de Ingenieros Agronomos, Universidad de Castilla-La 
      Mancha, Campus Universitario s/n, 02071 Albacete, Spain.
FAU - Cortes, Jose M
AU  - Cortes JM
FAU - Andini, Juan C
AU  - Andini JC
FAU - Vazquez, Ana
AU  - Vazquez A
FAU - Villen, Jesus
AU  - Villen J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120725
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Oils)
RN  - 0 (Sterols)
SB  - IM
MH  - Adsorption
MH  - Chromatography, Gas/*methods
MH  - Chromatography, Liquid/*methods
MH  - Oils/*chemistry
MH  - Sterols/*analysis
EDAT- 2012/08/14 06:00
MHDA- 2012/12/10 06:00
CRDT- 2012/08/14 06:00
PHST- 2012/04/27 00:00 [received]
PHST- 2012/07/12 00:00 [revised]
PHST- 2012/07/16 00:00 [accepted]
PHST- 2012/08/14 06:00 [entrez]
PHST- 2012/08/14 06:00 [pubmed]
PHST- 2012/12/10 06:00 [medline]
AID - S0021-9673(12)01112-0 [pii]
AID - 10.1016/j.chroma.2012.07.057 [doi]
PST - ppublish
SO  - J Chromatogr A. 2012 Sep 21;1256:191-6. doi: 10.1016/j.chroma.2012.07.057. Epub 
      2012 Jul 25.