PMID- 22885868
OWN - NLM
STAT- MEDLINE
DCOM- 20130809
LR  - 20211021
IS  - 1434-4726 (Electronic)
IS  - 0937-4477 (Linking)
VI  - 270
IP  - 3
DP  - 2013 Mar
TI  - Role of bacterial biofilms in idiopathic childhood epistaxis.
PG  - 909-14
LID - 10.1007/s00405-012-2132-2 [doi]
AB  - The objective of the study is to conduct a prospective trial investigating the 
      possible role of bacterial biofilms in the pathogenesis of severe idiopathic 
      childhood epistaxis. This study included 84 cases of severe idiopathic epistaxis, 
      aged below 16 years, who were prepared for cautery under general anesthesia. A 
      nasal swab was taken for bacterial culture and a nasal mucosal specimen (</= 3 
      mm(2)) was taken from the suspected site of bleeding just prior to cautery and 
      sent for bacterial identification by pathogen specific fluorescence in situ 
      hybridization (FISH) and also for detection of bacterial biofilms by scanning 
      electron microscope (SEM). Nasal mucosal specimens from 20 children of the same 
      age prepared for reduction of fracture nasal bones and have no nasal problems 
      were taken as a control group. Bacterial culture was positive in 27.3 % of 
      patients and the most common organism was Staphylococcus aureus (19 %). By SEM, 
      biofilm formation was detected in only six patients (7.1 %). Evaluation of nasal 
      specimens with FISH was positive for pathogenic bacteria in 37 % of cases; the 
      most common organism was S. aureus (22.6 % of cases). In the control group, no 
      biofilm was detected by SEM and no pathogenic bacteria were cultured or detected 
      by FISH. The difference between the two groups was statistically significant. 
      Bacterial biofilm does not seem to play a major role in the pathogenesis of 
      idiopathic epistaxis in children (only positive in 7.1 % of cases by SEM) 
      although a low-grade chronic inflammation is not infrequently present (37 % of 
      cases detected by FISH). FISH is more sensitive than bacterial culture in 
      detecting bacterial infections. S. aureus was the most common pathogen detected 
      by both techniques.
FAU - Saafan, Magdy Eisa
AU  - Saafan ME
AD  - Tanta University Hospitals, 61 Kafr Esam Street, El Nadi Square, Tanta, Egypt. 
      eisamagdy@hotmail.com
FAU - Ibrahim, Wesam Salah
AU  - Ibrahim WS
LA  - eng
PT  - Journal Article
DEP - 20120812
PL  - Germany
TA  - Eur Arch Otorhinolaryngol
JT  - European archives of oto-rhino-laryngology : official journal of the European 
      Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the 
      German Society for Oto-Rhino-Laryngology - Head and Neck Surgery
JID - 9002937
SB  - IM
MH  - Adolescent
MH  - Bacterial Infections/*complications
MH  - *Bacterial Physiological Phenomena
MH  - *Biofilms
MH  - Case-Control Studies
MH  - Child
MH  - Child, Preschool
MH  - Epistaxis/*etiology
MH  - Female
MH  - Haemophilus influenzae/physiology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Microscopy, Electron, Scanning
MH  - Moraxella catarrhalis/physiology
MH  - Nasal Mucosa/*microbiology
MH  - Prospective Studies
MH  - Staphylococcus aureus/physiology
MH  - Streptococcus pneumoniae/physiology
EDAT- 2012/08/14 06:00
MHDA- 2013/08/10 06:00
CRDT- 2012/08/14 06:00
PHST- 2012/05/04 00:00 [received]
PHST- 2012/07/18 00:00 [accepted]
PHST- 2012/08/14 06:00 [entrez]
PHST- 2012/08/14 06:00 [pubmed]
PHST- 2013/08/10 06:00 [medline]
AID - 10.1007/s00405-012-2132-2 [doi]
PST - ppublish
SO  - Eur Arch Otorhinolaryngol. 2013 Mar;270(3):909-14. doi: 
      10.1007/s00405-012-2132-2. Epub 2012 Aug 12.