PMID- 22886978
OWN - NLM
STAT- MEDLINE
DCOM- 20140909
LR  - 20221207
IS  - 1672-0733 (Print)
IS  - 1672-0733 (Linking)
VI  - 32
IP  - 4
DP  - 2012 Aug
TI  - Effect of miR-513a-5p on etoposide-stimulating B7-H1 expression in retinoblastoma 
      cells.
PG  - 601-606
LID - 10.1007/s11596-012-1004-8 [doi]
AB  - This study investigated the effect of etoposide, an anticancer chemotherapy drug, 
      on B7-H1 expression in retinoblastoma (Rb) cells and the role of miR-513a-5p in 
      the process. Rb cells were divided into control and etoposide groups. In the 
      etoposide group, cells were treated with etoposide at different concentrations 
      (2.5, 5, 10, 20 and 40 mug/mL) for 24 h. Those given no treatment of etopside 
      served as controls. Reverse transcription polymerase chain reaction (RT-PCR), 
      fluorescence quantitative PCR and flow cytometry were performed to measure the 
      mRNA and protein expression of B7-H1 in Rb cells. The mRNA expression of 
      miR-513a-5p in Rb cells before and after etoposide treatment was also detected by 
      fluorescence quantitative PCR. The miR-513a-5p mimics and the miR-513a-5p 
      inhibitor were transfected into Rb cells separately, and fluorescence 
      quantitative PCR and flow cytometry were used to detect the effect of the 
      miR-513a-5p mimics or inhibitor on B7-H1 expression. TargetScan5.2 was employed 
      to predict the miR-513a-5p binding sites in the 3'-untranslated region of B7-H1 
      mRNA. Luciferase reporter plasmids carrying this site were prepared and 
      transfected into Rb cells and luciferase activity analyzed. The results showed 
      that etoposide stimulated the mRNA and protein expression of B7-H1 in Rb cells, 
      which reached a maximal level after treatment with 5 mug/mL etoposide (P<0.05). 
      However, miR-513a-5p expression was decreased in Rb cells after etoposide 
      treatment. When the miR-513a-5p inhibitor was added, B7-H1 expression was 
      increased with the concentration of the miR-513a-5p inhibitor (P<0.05). Moreover, 
      B7-H1 expression was decreased gradually with the concentration of the 
      miR-513a-5p mimics increased (P<0.01). Additionally, the miR-513a-5p mimics were 
      found to inhibit the luciferase activity. It was concluded that etoposide can 
      promote B7-H1 expression in Rb cells, which may be associated with 
      chemoresistance. The promoting effect of etoposide on B7-H1 expression can be 
      reversed by miR-513a-5p mimics. MiR-513a-5p inhibits the mRNA and protein 
      expression of B7-H1 via binding to the 3'-UTR of B7-H1 mRNA.
FAU - Wu, Li
AU  - Wu L
AD  - Eye Center, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
FAU - Chen, Zhen
AU  - Chen Z
AD  - Eye Center, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
FAU - Zhang, Jian
AU  - Zhang J
AD  - Department of Ophthalmology, Ezhou Central Hospital, Ezhou, 436000, China.
FAU - Xing, Yiqiao
AU  - Xing Y
AD  - Eye Center, Renmin Hospital of Wuhan University, Wuhan, 430060, China. 
      xyqdr07@yahoo.com.cn.
LA  - eng
PT  - Journal Article
DEP - 20120811
PL  - China
TA  - J Huazhong Univ Sci Technolog Med Sci
JT  - Journal of Huazhong University of Science and Technology. Medical sciences = Hua 
      zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. 
      Yixue Yingdewen ban
JID - 101169627
RN  - 0 (B7-H1 Antigen)
RN  - 0 (CD274 protein, human)
RN  - 0 (MIRN513A1 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 6PLQ3CP4P3 (Etoposide)
SB  - IM
MH  - B7-H1 Antigen/*genetics
MH  - Cell Line, Tumor
MH  - Etoposide/*pharmacology
MH  - Gene Expression/*drug effects/genetics
MH  - Humans
MH  - MicroRNAs/*genetics
MH  - Retinoblastoma/*genetics
EDAT- 2012/08/14 06:00
MHDA- 2014/09/10 06:00
CRDT- 2012/08/14 06:00
PHST- 2012/01/16 00:00 [received]
PHST- 2012/08/14 06:00 [entrez]
PHST- 2012/08/14 06:00 [pubmed]
PHST- 2014/09/10 06:00 [medline]
AID - 10.1007/s11596-012-1004-8 [pii]
AID - 10.1007/s11596-012-1004-8 [doi]
PST - ppublish
SO  - J Huazhong Univ Sci Technolog Med Sci. 2012 Aug;32(4):601-606. doi: 
      10.1007/s11596-012-1004-8. Epub 2012 Aug 11.