PMID- 22886995
OWN - NLM
STAT- MEDLINE
DCOM- 20130422
LR  - 20220330
IS  - 1097-4644 (Electronic)
IS  - 0730-2312 (Linking)
VI  - 114
IP  - 1
DP  - 2013 Jan
TI  - The release of glutamate from cortical neurons regulated by BDNF via the 
      TrkB/Src/PLC-gamma1 pathway.
PG  - 144-51
LID - 10.1002/jcb.24311 [doi]
AB  - The brain-derived neurotrophic factor (BDNF) participates in the regulation of 
      cortical neurons by influencing the release of glutamate. However, the specific 
      mechanisms are unclear. Hence, we isolated and cultured the cortical neurons of 
      Sprague Dawley rats. Specific inhibitors of TrkB, Src, PLC-gamma1, Akt, and MEK1/2 
      (i.e., K252a, PP2, U73122, LY294002, and PD98059, respectively) were used to 
      treat cortical neurons and to detect the glutamate release from cortical neurons 
      stimulated with BDNF. BDNF significantly increased glutamate release, and 
      simultaneously enhanced phosphorylation levels of TrkB, Src, PLC-gamma, Akt, and 
      Erk1/2. For BDNF-stimulated cortical neurons, K252a inhibited glutamate release 
      and inhibited the phosphorylation levels of TrkB, Src, PLC-gamma, Erk1/2, and Akt (P 
      < 0.05). PP2 reduced the glutamate release from BDNF-stimulated cortical neurons 
      (P < 0.05) and inhibited the phosphorylation levels of TrkB and PLC-gamma1 (P < 
      0.05). However, PP2 had no effect on the phosphorylation levels of Erk1/2 or Akt 
      (P > 0.05). U73122 inhibited the glutamate release from BDNF-stimulated cortical 
      neurons, but had no influence on the phosphorylation levels of TrkB, Src, Erk1/2, 
      or Akt (P > 0.05). LY294002 and PD98059 did not affect the BDNF-stimulated 
      glutamate release and did not inhibit the phosphorylation levels of TrkB, Src, or 
      PLC-gamma1. In summary, BDNF stimulated the glutamate release from cortical neurons 
      via the TrkB/Src/PLC-gamma1 signaling pathway.
CI  - Copyright (c) 2012 Wiley Periodicals, Inc.
FAU - Zhang, Zitao
AU  - Zhang Z
AD  - Department of Spine Surgery, The First Affiliated Hospital of Nanjing Medical 
      University, Nanjing 210029, China.
FAU - Fan, Jin
AU  - Fan J
FAU - Ren, Yongxin
AU  - Ren Y
FAU - Zhou, Wei
AU  - Zhou W
FAU - Yin, Guoyong
AU  - Yin G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 0 (AG 1879)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Carbazoles)
RN  - 0 (Chromones)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Estrenes)
RN  - 0 (Flavonoids)
RN  - 0 (Indole Alkaloids)
RN  - 0 (Morpholines)
RN  - 0 (Pyrimidines)
RN  - 0 (Pyrrolidinones)
RN  - 112648-68-7 
      (1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione)
RN  - 31M2U1DVID (2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - 97161-97-2 (staurosporine aglycone)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - EC 2.7.10.2 (src-Family Kinases)
RN  - EC 3.1.4.3 (Phospholipase C gamma)
RN  - SJE1IO5E3I (2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/antagonists & inhibitors/genetics/*metabolism
MH  - Carbazoles/pharmacology
MH  - Cerebral Cortex/cytology/drug effects/*metabolism
MH  - Chromones/pharmacology
MH  - Embryo, Mammalian
MH  - Enzyme Inhibitors/pharmacology
MH  - Estrenes/pharmacology
MH  - Flavonoids/pharmacology
MH  - Gene Expression Regulation/drug effects
MH  - Glutamic Acid/biosynthesis/*metabolism
MH  - Indole Alkaloids/pharmacology
MH  - Morpholines/pharmacology
MH  - Neurons/cytology/drug effects/*metabolism
MH  - Phospholipase C gamma/antagonists & inhibitors/genetics/metabolism
MH  - Phosphorylation/drug effects
MH  - Primary Cell Culture
MH  - Pyrimidines/pharmacology
MH  - Pyrrolidinones/pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptor, trkB/antagonists & inhibitors/genetics/metabolism
MH  - Signal Transduction/drug effects
MH  - src-Family Kinases/antagonists & inhibitors/genetics/metabolism
EDAT- 2012/08/14 06:00
MHDA- 2013/04/23 06:00
CRDT- 2012/08/14 06:00
PHST- 2012/05/25 00:00 [received]
PHST- 2012/07/26 00:00 [accepted]
PHST- 2012/08/14 06:00 [entrez]
PHST- 2012/08/14 06:00 [pubmed]
PHST- 2013/04/23 06:00 [medline]
AID - 10.1002/jcb.24311 [doi]
PST - ppublish
SO  - J Cell Biochem. 2013 Jan;114(1):144-51. doi: 10.1002/jcb.24311.