PMID- 22888751
OWN - NLM
STAT- MEDLINE
DCOM- 20120906
LR  - 20211203
IS  - 1550-7033 (Print)
IS  - 1550-7033 (Linking)
VI  - 8
IP  - 5
DP  - 2012 Oct
TI  - Preparation and characterization of magnetically responsive bacterial polyester 
      based nanospheres for cancer therapy.
PG  - 800-8
AB  - Polyhydroxyalkanoates (PHA) are natural, thermoplastic polyesters and due to 
      their biocompatible and biodegradable properties they are good alternatives for 
      the production of scaffolds for engineered tissues or nanoparticles for drug 
      delivery. As a member of polyhydroxyalkanoate family, polyhydroxybutyrates (PHB) 
      have been widely used as a biomaterial for in vitro and in vivo studies since 
      their mechanical properties are very similar to conventional plastics. By using 
      multi-emulsion technique, iron oxide particles were coated with 
      polyhydroxybutyrate (PHB) polymer synthesized from Alcaligenes eutrophus bacteria 
      and the magnetic carrier system was prepared accordingly. The bare nanoparticles 
      and magnetic nanoparticles were morphologically, structurally and magnetically 
      characterized by using Scanning electron microscope (SEM) and Atomic force 
      microscope (AFM); Fourier Transform Infrared Spectrometry (FTIR), and Electron 
      Spin Resonance (ESR) and Vibrating Sample Magnetometer (VSM) techniques, 
      respectively. Particle size of PHB nanoparticles was determined by Zeta Sizer. It 
      was found that the smallest particles were in the range of 239.43 +/- 5.25 nm in 
      diameter. Concanavalin-A (Con-A) was used for targeting the cancer cells while 
      etoposide was used as drug. Con-A and etoposide were loaded onto the particles. 
      Release studies of etoposide were evaluated and the system was optimized for the 
      further in vivo applications. Finally different formulation magnetic PHB 
      nanoparticles cytotoxicity were evaluated in cell culture studies and used HeLa 
      cell line (cervical cancer cells) as a cancer cells and L929 cells (mouse 
      fibroblast cells) as a non-cancer cell line.
FAU - Erdal, Ebru
AU  - Erdal E
AD  - Hacettepe University, Nanotechnology and Nanomedicine Division, Beytepe, 06800 
      Ankara, Turkey.
FAU - Kavaz, Doga
AU  - Kavaz D
FAU - Sam, Mesut
AU  - Sam M
FAU - Demirbilek, Murat
AU  - Demirbilek M
FAU - Demirbilek, Melike Erol
AU  - Demirbilek ME
FAU - Saglam, Necdet
AU  - Saglam N
FAU - Denkbas, Emir Baki
AU  - Denkbas EB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biomed Nanotechnol
JT  - Journal of biomedical nanotechnology
JID - 101230869
RN  - 0 (Antineoplastic Agents, Phytogenic)
RN  - 0 (Magnetite Nanoparticles)
RN  - 0 (Nanocapsules)
RN  - 0 (PHB protein, human)
RN  - 0 (Polyhydroxyalkanoates)
RN  - 0 (Prohibitins)
RN  - 11028-71-0 (Concanavalin A)
RN  - 6PLQ3CP4P3 (Etoposide)
SB  - IM
MH  - Antineoplastic Agents, Phytogenic/administration & dosage/chemistry
MH  - Cell Survival/*drug effects
MH  - Concanavalin A/administration & dosage/chemistry/*pharmacokinetics
MH  - Cupriavidus necator/*metabolism
MH  - Etoposide/*administration & dosage
MH  - HeLa Cells
MH  - Humans
MH  - Magnetite Nanoparticles/*administration & dosage/chemistry
MH  - Materials Testing
MH  - Nanocapsules/*administration & dosage/chemistry
MH  - Nanospheres/administration & dosage/chemistry
MH  - Polyhydroxyalkanoates/*chemistry
MH  - Prohibitins
EDAT- 2012/08/15 06:00
MHDA- 2012/09/07 06:00
CRDT- 2012/08/15 06:00
PHST- 2012/08/15 06:00 [entrez]
PHST- 2012/08/15 06:00 [pubmed]
PHST- 2012/09/07 06:00 [medline]
PST - ppublish
SO  - J Biomed Nanotechnol. 2012 Oct;8(5):800-8.