PMID- 22889831
OWN - NLM
STAT- MEDLINE
DCOM- 20130411
LR  - 20121130
IS  - 1530-6860 (Electronic)
IS  - 0892-6638 (Linking)
VI  - 26
IP  - 12
DP  - 2012 Dec
TI  - Interaction of HLA-DR and CD74 at the cell surface of antigen-presenting cells by 
      single particle image analysis.
PG  - 4886-96
LID - 10.1096/fj.12-211466 [doi]
AB  - Major histocompatibility complex (MHC) class II-associated antigen presentation 
      involves an array of interacting molecules. CD74, the cell surface isoform of the 
      MHC class II-associated invariant chain, is one such molecule; its role remains 
      poorly defined. To address this, we have employed a high-resolution 
      single-particle imaging method for quantifying the colocalization of CD74 with 
      human leukocyte antigen (HLA)-DR molecules on human fibroblast cells known for 
      their capacity to function as antigen-presenting cells. We have also examined 
      whether the colocalization induces internalization of HLA-DR using HA(307-319), a 
      "universal" peptide that binds specifically to the peptide-binding groove of all 
      HLA-DR molecules, irrespective of their alleles. We have determined that 25 +/- 
      1.3% of CD74 and 17 +/- 0.3% of HLA-DR are colocalized, and the association of CD74 
      with HLA-DR and the internalization of HLA-DR are both inhibited by HA(307-319). 
      A similar inhibition of HLA-DR internalization was observed in freshly isolated 
      monocyte-derived dendritic cells. A key role of CD74 is to translocate HLA-DR 
      molecules to early endosomes for reloading with peptides prior to recycling to 
      the cell surface. We conclude that CD74 regulates the balance of peptide-occupied 
      and peptide-free forms of MHC class II at the cell surface.
FAU - Karakikes, Ioannis
AU  - Karakikes I
AD  - School of Biological Sciences, University of Essex, Wivenhoe Park, Colchester C04 
      3SQ, UK.
FAU - Morrison, Ian E G
AU  - Morrison IE
FAU - O'Toole, Peter
AU  - O'Toole P
FAU - Metodieva, Gergana
AU  - Metodieva G
FAU - Navarrete, Cristina V
AU  - Navarrete CV
FAU - Gomez, Jesus
AU  - Gomez J
FAU - Miranda-Sayago, Jose M
AU  - Miranda-Sayago JM
FAU - Cherry, Richard J
AU  - Cherry RJ
FAU - Metodiev, Metodi
AU  - Metodiev M
FAU - Fernandez, Nelson
AU  - Fernandez N
LA  - eng
GR  - Biotechnology and Biological Sciences Research Council/United Kingdom
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120813
PL  - United States
TA  - FASEB J
JT  - FASEB journal : official publication of the Federation of American Societies for 
      Experimental Biology
JID - 8804484
RN  - 0 (Antigens, Differentiation, B-Lymphocyte)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Hemagglutinin Glycoproteins, Influenza Virus)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Peptide Fragments)
RN  - 0 (influenza hemagglutinin (307-319))
RN  - 0 (invariant chain)
SB  - IM
MH  - Adult
MH  - Algorithms
MH  - Antigen-Presenting Cells/*metabolism
MH  - Antigens, Differentiation, B-Lymphocyte/genetics/*metabolism
MH  - Cell Line
MH  - Cell Membrane/*metabolism
MH  - Cells, Cultured
MH  - Dendritic Cells/metabolism
MH  - Endocytosis/drug effects
MH  - Flow Cytometry
MH  - Fluorescence Resonance Energy Transfer
MH  - HLA-DR Antigens/genetics/*metabolism
MH  - Hemagglutinin Glycoproteins, Influenza Virus/metabolism/pharmacology
MH  - Histocompatibility Antigens Class II/genetics/*metabolism
MH  - Humans
MH  - Imaging, Three-Dimensional
MH  - Microscopy, Confocal
MH  - Microscopy, Fluorescence/*methods
MH  - Peptide Fragments/metabolism/pharmacology
MH  - Protein Binding/drug effects
EDAT- 2012/08/15 06:00
MHDA- 2013/04/12 06:00
CRDT- 2012/08/15 06:00
PHST- 2012/08/15 06:00 [entrez]
PHST- 2012/08/15 06:00 [pubmed]
PHST- 2013/04/12 06:00 [medline]
AID - fj.12-211466 [pii]
AID - 10.1096/fj.12-211466 [doi]
PST - ppublish
SO  - FASEB J. 2012 Dec;26(12):4886-96. doi: 10.1096/fj.12-211466. Epub 2012 Aug 13.