PMID- 22889984
OWN - NLM
STAT- MEDLINE
DCOM- 20130722
LR  - 20191224
IS  - 1536-4828 (Electronic)
IS  - 0885-3177 (Linking)
VI  - 42
IP  - 2
DP  - 2013 Mar
TI  - Connexins regulate cell functions in pancreatic stellate cells.
PG  - 308-16
LID - 10.1097/MPA.0b013e31825c51d6 [doi]
AB  - OBJECTIVES: Pancreatic stellate cells (PSCs) play a pivotal role in pancreatic 
      fibrosis associated with chronic pancreatitis and pancreatic cancer. Connexins 
      (Cxs) allow direct intercellular communications as components of gap junction but 
      also play important roles in the regulation of cell proliferation, cell 
      differentiation, and tissue development. We here examined the expression of Cxs 
      and Cx-mediated regulation of cell functions in PSCs. METHODS: Human PSCs were 
      isolated from patients undergoing operation for chronic pancreatitis or 
      pancreatic cancer. The expression of Cxs was examined by reverse transcription 
      polymerase chain reaction, Western blotting, and immunofluorescent staining. The 
      roles of Cxs in PSC functions were examined by using carbenoxolone, a 
      broad-spectrum Cx inhibitor, and small interfering RNA for Cx43. RESULTS: Human 
      activated PSCs expressed a variety of Cxs including Cx43 both in vitro and in 
      vivo. Carbenoxolone inhibited platelet-derived growth factor-BB-induced 
      proliferation and migration, and type I collagen expression in PSCs. In addition, 
      carbenoxolone inhibited the activation of quiescent PSCs to a myofibroblastlike 
      phenotype. Decreased Cx43 expression by small interfering RNA resulted in 
      decreased proliferation and type I collagen expression. CONCLUSIONS: Pancreatic 
      stellate cells expressed a variety of Cxs. Connexins, especially Cx43, might 
      regulate the cell functions and activation of PSCs.
FAU - Masamune, Atsushi
AU  - Masamune A
AD  - Division of Gastroenterology, Tohoku University Graduate School of Medicine, 
      Sendai, Japan. amasamune@med.tohoku.ac.jp
FAU - Suzuki, Noriaki
AU  - Suzuki N
FAU - Kikuta, Kazuhiro
AU  - Kikuta K
FAU - Ariga, Hiroyuki
AU  - Ariga H
FAU - Hayashi, Shintaro
AU  - Hayashi S
FAU - Takikawa, Tetsuya
AU  - Takikawa T
FAU - Kume, Kiyoshi
AU  - Kume K
FAU - Hamada, Shin
AU  - Hamada S
FAU - Hirota, Morihisa
AU  - Hirota M
FAU - Kanno, Atsushi
AU  - Kanno A
FAU - Egawa, Shinichi
AU  - Egawa S
FAU - Unno, Michiaki
AU  - Unno M
FAU - Shimosegawa, Tooru
AU  - Shimosegawa T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Pancreas
JT  - Pancreas
JID - 8608542
RN  - 0 (Collagen Type I)
RN  - 0 (Connexin 43)
RN  - 0 (Connexins)
RN  - 0 (GJA1 protein, human)
RN  - 0 (Gja1 protein, rat)
RN  - 0 (Proto-Oncogene Proteins c-sis)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Small Interfering)
RN  - 1B56C968OA (Becaplermin)
RN  - MM6384NG73 (Carbenoxolone)
SB  - IM
MH  - Animals
MH  - Becaplermin
MH  - Blotting, Western
MH  - Carbenoxolone/pharmacology
MH  - Cell Movement
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Collagen Type I/metabolism
MH  - Connexin 43/metabolism
MH  - Connexins/antagonists & inhibitors/genetics/*metabolism
MH  - Dose-Response Relationship, Drug
MH  - Fibrosis
MH  - Fluorescent Antibody Technique
MH  - Humans
MH  - Male
MH  - Myofibroblasts/metabolism/pathology
MH  - Pancreatic Neoplasms/genetics/*metabolism/pathology
MH  - Pancreatic Stellate Cells/drug effects/*metabolism/pathology
MH  - Pancreatitis, Chronic/genetics/*metabolism/pathology
MH  - Proto-Oncogene Proteins c-sis/metabolism
MH  - RNA Interference
MH  - RNA, Messenger/metabolism
MH  - RNA, Small Interfering/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Real-Time Polymerase Chain Reaction
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2012/08/15 06:00
MHDA- 2013/07/23 06:00
CRDT- 2012/08/15 06:00
PHST- 2012/08/15 06:00 [entrez]
PHST- 2012/08/15 06:00 [pubmed]
PHST- 2013/07/23 06:00 [medline]
AID - 10.1097/MPA.0b013e31825c51d6 [doi]
PST - ppublish
SO  - Pancreas. 2013 Mar;42(2):308-16. doi: 10.1097/MPA.0b013e31825c51d6.