PMID- 22890077
OWN - NLM
STAT- MEDLINE
DCOM- 20130501
LR  - 20220408
IS  - 1873-7544 (Electronic)
IS  - 0306-4522 (Linking)
VI  - 223
DP  - 2012 Oct 25
TI  - Chronic restraint stress decreases glial fibrillary acidic protein and glutamate 
      transporter in the periaqueductal gray matter.
PG  - 209-18
LID - S0306-4522(12)00827-5 [pii]
LID - 10.1016/j.neuroscience.2012.08.007 [doi]
AB  - Stress affects brain activity and promotes long-term changes in multiple neural 
      systems. Exposure to stressors causes substantial effects on the perception and 
      response to pain. In several animal models, chronic stress produces lasting 
      hyperalgesia. Postmortem studies of stress-related psychiatric disorders have 
      demonstrated a decrease in the number of astrocytes and the level of glial 
      fibrillary acidic protein (GFAP), a marker for astrocyte, in the cerebral cortex. 
      Since astrocytes play vital roles in maintaining neuroplasticity via synapse 
      maintenance and secretion of neurotrophins, impairment of astrocytes is thought 
      to be involved in the neuropathology. In the present study we examined GFAP and 
      excitatory amino acid transporter 2 (EAAT2) protein levels in the periaqueductal 
      gray matter (PAG) after subacute and chronic restraint stresses to clarify 
      changes in descending pain modulatory system in the rat with stress-induced 
      hyperalgesia. Chronic restraint stress (6h/day for 3 weeks), but not subacute 
      restraint stress (6h/day for 3 days), caused a marked mechanical hypersensitivity 
      and aggressive behavior. The chronic restraint stress induced a significant 
      decrease of GFAP protein level in the PAG (32.0 +/- 8.9% vs. control group, 
      p<0.05). In immunohistochemical analysis the remarkable decrease of GFAP was 
      observed in the ventrolateral PAG. The EAAT2 protein level in the 3 weeks stress 
      group (79.6 +/- 6.8%) was significantly lower compared to that in the control group 
      (100.0 +/- 6.1%, p<0.05). In contrast there was no significant difference in the 
      GFAP and EAAT2 protein levels between the control and 3 days stress groups These 
      findings suggest a dysfunction of the PAG that plays pivotal roles in the 
      organization of strategies for coping with stressors and in pain modulation after 
      chronic restraint stress.
CI  - Copyright (c) 2012 IBRO. Published by Elsevier Ltd. All rights reserved.
FAU - Imbe, H
AU  - Imbe H
AD  - Department of Physiology, Wakayama Medical University, Kimiidera 811-1, Wakayama 
      City 641-8509, Japan. imbe@wakayama-med.ac.jp
FAU - Kimura, A
AU  - Kimura A
FAU - Donishi, T
AU  - Donishi T
FAU - Kaneoke, Y
AU  - Kaneoke Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120810
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Amino Acid Transport System X-AG)
RN  - 0 (Glial Fibrillary Acidic Protein)
SB  - IM
MH  - Aggression/psychology
MH  - Amino Acid Transport System X-AG/*metabolism
MH  - Animals
MH  - Gene Expression Regulation/*physiology
MH  - Glial Fibrillary Acidic Protein/*metabolism
MH  - Hyperalgesia/physiopathology
MH  - Male
MH  - Pain Threshold/physiology
MH  - Periaqueductal Gray/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - *Restraint, Physical
MH  - Synaptosomes/metabolism
MH  - Time Factors
EDAT- 2012/08/15 06:00
MHDA- 2013/05/02 06:00
CRDT- 2012/08/15 06:00
PHST- 2012/03/30 00:00 [received]
PHST- 2012/08/01 00:00 [revised]
PHST- 2012/08/02 00:00 [accepted]
PHST- 2012/08/15 06:00 [entrez]
PHST- 2012/08/15 06:00 [pubmed]
PHST- 2013/05/02 06:00 [medline]
AID - S0306-4522(12)00827-5 [pii]
AID - 10.1016/j.neuroscience.2012.08.007 [doi]
PST - ppublish
SO  - Neuroscience. 2012 Oct 25;223:209-18. doi: 10.1016/j.neuroscience.2012.08.007. 
      Epub 2012 Aug 10.