PMID- 22892046
OWN - NLM
STAT- MEDLINE
DCOM- 20130308
LR  - 20121010
IS  - 1600-0897 (Electronic)
IS  - 1046-7408 (Linking)
VI  - 68
IP  - 5
DP  - 2012 Nov
TI  - Trophoblastic cell lines ACH1P and AC-1M32 react with a distinctive cytokine 
      pattern toward Listeria monocytogenes and show morphologic differences.
PG  - 387-91
LID - 10.1111/aji.12004 [doi]
AB  - PROBLEM: The differential reaction of cytotrophoblast and syncytiotrophoblast 
      towards infection with listeria monocytogenes (LM) is pivotal to its 
      pathogenicity. In this study we tested the cytokine signature upon infection with 
      listeria monocytogenes (LM) in an in vitro model. METHOD OF STUDY: We compared 
      two related trophoblastic cell lines (AC-1M32 and ACH1P). The cell line ACH1P 
      showed syncytium formation, whereas AC-1M32 did not fuse, as demonstrated with 
      immunfluorescence E-Cadherin staining. In a Multi-Analyte ELISArray we tested for 
      concentrations of TNFalpha, IL-1beta, IL-2, IL-4, IL-6, IL-10, IL-12, IL-13, IL-17A, 
      IL-8, MCP1, MIP-1a, MIP-1b, MDC, Eotaxin, IFNgamma, G-CSF, TGFbeta1 after 8 and 24 
      hours. RESULTS: Compared to unstimulated cells, the syncytial cell line ACH1P 
      showed a significant induction of Interleukin-6 (IL-6), Monocyte Chemotactic 
      Protein-1 (MCP-1) and transforming growth factor beta-1 (TGFbeta1). Incubating AC-1M32 
      with LM, however, showed significantly reduced IL-6 levels and a massively 
      increased (~300fold) TGFbeta1 secretion compared to unstimulated controls. 
      CONCLUSIONS: A functional anti-LM immune response was only induced by the 
      syncytium-forming cell line ACH1P. Using the two sister cell lines AC-1M32 and 
      ACH1P in an in vitro LM-stimulation assay might facilitate research in the area 
      of placental listeria infection.
CI  - (c) 2012 John Wiley & Sons A/S.
FAU - Santoso, Laura
AU  - Santoso L
AD  - Department of Obstetrics and Gynecology, Ludwig-Maximilians University, Munich, 
      Germany.
FAU - Friese, Klaus
AU  - Friese K
FAU - Jeschke, Udo
AU  - Jeschke U
FAU - Scholz, Christoph
AU  - Scholz C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120814
PL  - Denmark
TA  - Am J Reprod Immunol
JT  - American journal of reproductive immunology (New York, N.Y. : 1989)
JID - 8912860
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-6)
RN  - 0 (Transforming Growth Factor beta)
SB  - IM
MH  - Cell Line
MH  - Chemokine CCL2/metabolism
MH  - Cytokines/*biosynthesis
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - Giant Cells/*ultrastructure
MH  - Humans
MH  - Interleukin-6/metabolism
MH  - Listeria monocytogenes/immunology/*pathogenicity
MH  - Listeriosis/*immunology/microbiology
MH  - Transforming Growth Factor beta/metabolism
MH  - Trophoblasts/*immunology/*microbiology/ultrastructure
EDAT- 2012/08/16 06:00
MHDA- 2013/03/09 06:00
CRDT- 2012/08/16 06:00
PHST- 2012/03/22 00:00 [received]
PHST- 2012/07/10 00:00 [accepted]
PHST- 2012/08/16 06:00 [entrez]
PHST- 2012/08/16 06:00 [pubmed]
PHST- 2013/03/09 06:00 [medline]
AID - 10.1111/aji.12004 [doi]
PST - ppublish
SO  - Am J Reprod Immunol. 2012 Nov;68(5):387-91. doi: 10.1111/aji.12004. Epub 2012 Aug 
      14.